Soubasis Nektarios, Rallis Timoleon S, Vlemmas John, Adamama-Moraitou Katerina K, Roubies Nikolaos, Prassinos Nikitas N, Brellou Georgia
Clinic of Companion Animal Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Gastroenterol Hepatol. 2006 Mar;21(3):599-604. doi: 10.1111/j.1440-1746.2005.04066.x.
Iron (Fe) status is altered in human and experimental animal hepatopathies. In dogs limited data are available. The aim of this study was to investigate serum iron (SI), total iron binding capacity (TIBC), percentage transferrin saturation (SAT) and Fe status in the liver of dogs with experimentally induced hepatopathy.
Fourteen 1-year-old dogs were divided into two equal groups. In order for hepatopathy to be induced, 0.25 mL/kg body weight of carbon tetrachloride (CCl4) solution was administered once daily, orally, for a 10-week period in group B dogs, while group A dogs were used as controls. SI, TIBC and SAT values were measured 3 times before the beginning (baseline value) and 10 times at weekly intervals during the experiment. Liver samples, obtained before the administration of CCl4 and at the end of the experimental period (10 weeks), were subjected to Fe determination, as well as to histopathological and histochemical analysis.
At the end of the experiment SI, TIBC and liver iron concentration, as well as liver total iron score were significantly increased in group B dogs. Distribution of granular hemosiderin iron in hepatocytes, Kupffer cells, and portal triads was noticed. Positive correlations were found between SI and liver Fe concentration, as well as histochemically determined Fe. Moreover, positive correlations were evident between liver fibrosis and serum, as well as liver Fe values.
Experimentally induced chronic hepatopathy in dogs causes Fe status disturbances. Increased serum and liver iron concentration produces liver histopathological deterioration and it may be worth attention during laboratory evaluation in canine hepatopathy.
在人类和实验动物肝病中，铁（Fe）状态会发生改变。关于犬类的相关数据有限。本研究旨在调查实验性诱导肝病犬的血清铁（SI）、总铁结合力（TIBC）、转铁蛋白饱和度百分比（SAT）以及肝脏铁状态。
将14只1岁的犬分为两组，每组数量相等。为诱导肝病，B组犬每天口服0.25 mL/kg体重的四氯化碳（CCl4）溶液，持续10周，而A组犬作为对照。在实验开始前（基线值）测量3次SI、TIBC和SAT值，并在实验期间每周测量10次。在给予CCl4之前和实验期结束时（10周）采集肝脏样本，进行铁测定以及组织病理学和组织化学分析。
实验结束时，B组犬的SI、TIBC、肝脏铁浓度以及肝脏总铁评分均显著升高。在肝细胞、库普弗细胞和门三联管中发现了颗粒状含铁血黄素铁的分布。SI与肝脏铁浓度以及组织化学测定的铁之间存在正相关。此外，肝脏纤维化与血清以及肝脏铁值之间也存在明显的正相关。
实验性诱导的犬慢性肝病会导致铁状态紊乱。血清和肝脏铁浓度升高会导致肝脏组织病理学恶化，在犬肝病的实验室评估中可能值得关注。
