人血清低密度脂蛋白的中子散射研究。
Neutron scattering study of human serum low density lipoprotein.
作者信息
Stuhrmann H B, Tardieu A, Mateu L, Sardet C, Luzzati V, Aggerbeck L, Scanu A M
出版信息
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2270-3. doi: 10.1073/pnas.72.6.2270.
Abstract
Regions of different proton density in the low density lipoprotein (LDL) particle from human serum have been determined by neutron scattering. From measurements in various H2O/D2O mixtures, the LDL particle appears to be quasi-spherical, with the centers of gravity of the hydrocarbon and polar regions coinciding. The average volume occupied by a particle was found to be 3.2 X 10-6 A-3, with the volume fraction occupied by the hydrocarbons being 60%. The ratius of gyration of the hydrocarbon region was 64 A, while that of the polar region was 100 A. consequently, the core of LDL is predominantly occupied by the hydrocarbon chains, while the outer shell is sparsely occupied by protein emerging from the lipid core.
摘要
通过中子散射已确定了来自人血清的低密度脂蛋白（LDL）颗粒中不同质子密度的区域。从在各种H₂O/D₂O混合物中的测量结果来看，LDL颗粒似乎是准球形的，碳氢化合物区域和极性区域的重心重合。发现一个颗粒占据的平均体积为3.2×10⁻⁶ Å⁻³，其中碳氢化合物所占的体积分数为60%。碳氢化合物区域的回转半径为64 Å，而极性区域的回转半径为100 Å。因此，LDL的核心主要由碳氢链占据，而外壳则稀疏地被从脂质核心伸出的蛋白质占据。
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