钙连蛋白的鉴定，一种由珠母贝外套膜特异性表达的钙结合蛋白。
Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera.
作者信息
Duplat D, Puisségur M, Bédouet L, Rousseau M, Boulzaguet H, Milet C, Sellos D, Van Wormhoudt A, Lopez E
机构信息
USM 0401, UMR CNRS 5178 Biologie des Organismes Marins et Ecosystèmes, Muséum National d'Histoire Naturelle, 7, rue Cuvier, 75005 Paris, France.
出版信息
FEBS Lett. 2006 May 1;580(10):2435-41. doi: 10.1016/j.febslet.2006.03.077. Epub 2006 Apr 7.
Nacre or mother-of-pearl in the shell of Pinctada margaritifera is composed of 95-99% calcium carbonate and 1-5% organic matrix. In this study, we developed an original technique to characterize the genes differentially expressed in nacre-forming cells (NFC) by combining suppression subtractive hybridization (SSH), to establish a cDNA subtractive library, with rapid amplification of cDNA ends (RACE)-PCR. Seventy-two specific cDNA sequences have been obtained so far. These include a protein containing two EF-hand Ca2+-binding domains which was completely sequenced after amplification by RACE-PCR. Its specific expression as well as the specificity of the SSH method was confirmed by semi-quantitative RT-PCR on NFC and mantle cells.
马氏珠母贝（Pinctada margaritifera）贝壳中的珍珠层或珍珠母由95 - 99%的碳酸钙和1 - 5%的有机基质组成。在本研究中，我们开发了一种原创技术，通过将抑制性消减杂交（SSH）与cDNA末端快速扩增（RACE）-PCR相结合来表征在珍珠层形成细胞（NFC）中差异表达的基因，以建立一个cDNA消减文库。到目前为止，已经获得了72个特异性cDNA序列。其中包括一种含有两个EF手型Ca2+结合结构域的蛋白质，该蛋白质在通过RACE-PCR扩增后进行了完全测序。通过对NFC和外套膜细胞进行半定量RT-PCR，证实了其特异性表达以及SSH方法的特异性。
Zotero 插件