School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, China.
Sci Rep. 2022 Mar 18;12(1):4743. doi: 10.1038/s41598-022-08610-5.
The shells of window pane oyster Placuna placenta are very thin and exhibit excellent optical transparency and mechanical robustness. However, little is known about the biomineralization-related proteins of the shells of P. placenta. In this work, we report the comprehensive transcriptome of the mantle tissue of P. placenta for the first time. The unigenes of the mantle tissue of P. placenta were annotated by using the public databases such as nr, GO, KOG, KEGG, and Pfam. 24,343 unigenes were annotated according to Pfam database, accounting for 21.48% of the total unigenes. We find that half of the annotated unigenes of the mantle tissue of P. placenta are consistent to the annotated unigenes from pacific oyster Crassostrea gigas according to nr database. The unigene sequence analysis from the mantle tissue of P. placenta indicates that 465,392 potential single nucleotide polymorphisms (SNPs) and 62,103 potential indel markers were identified from 60,371 unigenes. 178 unigenes of the mantle tissue of P. placenta are found to be homologous to those reported proteins related to the biomineralization process of molluscan shells, while 18 of them are highly expressed unigenes in the mantle tissue. It is proposed that four unigenes with the highest expression levels in the mantle tissue are very often related to the biomineralization process, while another three unigenes are potentially related to the biomineralization process according to the Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) analysis. In summary, the transcriptome analysis of the mantle tissue of P. Placenta shows the potential biomineralization-related proteins and this work may shed light for the shell formation mechanism of bivalves.
窗孔贻贝 Placuna placenta 的贝壳非常薄，具有出色的光学透明度和机械强度。然而，人们对 P. placenta 贝壳的生物矿化相关蛋白知之甚少。在这项工作中，我们首次报道了 P. placenta 套膜组织的综合转录组。P. placenta 套膜组织的 unigenes 通过 nr、GO、KOG、KEGG 和 Pfam 等公共数据库进行注释。根据 Pfam 数据库注释，有 24343 个 unigenes，占总 unigenes的 21.48%。我们发现，根据 nr 数据库，P. placenta 套膜组织中一半的注释 unigenes与太平洋牡蛎 Crassostrea gigas 的注释 unigenes一致。从 P. placenta 套膜组织的 unigene 序列分析中，从 60371 个 unigenes中鉴定出 465392 个潜在单核苷酸多态性（SNP）和 62103 个潜在插入缺失标记。从 P. placenta 套膜组织中发现 178 个 unigenes与报道的与软体动物贝壳生物矿化过程相关的蛋白质同源，其中 18 个是套膜组织中高表达的 unigenes。根据定量实时聚合酶链反应（qRT-PCR）分析，提出 4 个在套膜组织中表达水平最高的 unigenes与生物矿化过程非常相关，而另外 3 个 unigenes可能与生物矿化过程有关。综上所述，P. placenta 套膜组织的转录组分析显示了潜在的生物矿化相关蛋白，这项工作可能为双壳类贝壳形成机制提供了启示。
