Arrais-Silva Wagner Welber, Pinto Eduardo Fonseca, Rossi-Bergmann Bartira, Giorgio Selma
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, Caixa Postal 6109, CEP 13083-970, Campinas, São Paulo, Brazil.
Acta Trop. 2006 May;98(2):130-6. doi: 10.1016/j.actatropica.2006.03.001. Epub 2006 Apr 25.
In this study we determined whether exposing mice to hyperbaric oxygen (HBO) would alter various disease parameters of a susceptible mouse strain infected with Leishmania amazonensis. BALB/c mice exposed to HBO (100% O2 at a pressure of 2.5 ATA, 1h before parasite inoculation and subsequently for 20 days) showed significant delay in lesion development and reduction in lesion parasite burdens compared with HBO-unexposed mice. Circulating levels of interferon gamma (IFN-gamma) and tumor necrosis factor (TNF-alpha) were significantly elevated in HBO-exposed as compared to HBO-unexposed mice. Concanavalin A-stimulated lymph nodes cultures from HBO-exposed mice released significantly more IFN-gamma and less interleukin 10 (IL-10) than cultures from HBO-unexposed mice, consistent with a skewed Th1 response. These results demonstrate, for the first time, that HBO can play a pathogen control role during leishmaniasis. Further studies are needed to elucidate whether hyperoxia alone or increased atmospheric pressure alone can exert a similar effect.
在本研究中，我们确定了将小鼠暴露于高压氧（HBO）是否会改变感染亚马逊利什曼原虫的易感小鼠品系的各种疾病参数。与未暴露于HBO的小鼠相比，暴露于HBO（在接种寄生虫前1小时以2.5 ATA的压力给予100%氧气，随后持续20天）的BALB/c小鼠的病变发展显著延迟，病变中的寄生虫负荷降低。与未暴露于HBO的小鼠相比，暴露于HBO的小鼠循环中的干扰素γ（IFN-γ）和肿瘤坏死因子（TNF-α）水平显著升高。与未暴露于HBO的小鼠的培养物相比，来自暴露于HBO的小鼠的刀豆球蛋白A刺激的淋巴结培养物释放出显著更多的IFN-γ和更少的白细胞介素10（IL-10），这与偏向Th1的反应一致。这些结果首次证明，HBO在利什曼病期间可发挥病原体控制作用。需要进一步研究以阐明单独的高氧或单独的大气压升高是否能产生类似的效果。
