Liou Jer-Young, Shyu Kou-Gi, Lu Ming-Jen, Chao Hung-Hsin, Wang Bao-Wei, Kuan Pei-Liang
Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
J Formos Med Assoc. 2006 May;105(5):377-83. doi: 10.1016/S0929-6646(09)60133-9.
BACKGROUND/PURPOSE: Vascular endothelial growth factor (VEGF) and endostatin are related to ischemic heart disease. This study investigated pericardial fluid and serum levels of VEGF and endostatin in patients with or without ischemic heart disease.
A total of 39 patients (24 patients in the CAD group with significant coronary artery disease; 15 patients in the non-CAD group without coronary artery disease) undergoing open heart surgery were enrolled. In the CAD group, patients were classified according to good coronary collateralization (Group A; n = 11) or poor coronary collateralization (Group B; n = 13). Pericardial fluid and serum samples were obtained at the time of surgery. VEGF and endostatin were measured by enzyme-linked immunosorbent assay.
The levels of endostatin in both serum and pericardial fluid were significantly lower in the CAD group than in the non-CAD group (130.5 +/- 37.3 ng/mL vs. 172.4 +/- 37.8 ng/mL and 119.0 +/- 25.0 ng/mL vs. 143.0 +/- 23.5 ng/mL). The concentration of serum VEGF in the CAD group (92.6 +/- 18.2 pg/mL) was significantly higher than that in the non-CAD group (75.2 +/- 22.3 pg/mL). The concentration of serum VEGF in Group A (100.1 +/- 20.7 pg/mL) was significantly higher than that in Group B (84.3 +/- 12.4 pg/mL). The levels of pericardial fluid VEGF, serum and pericardial fluid endostatin were not significantly different between Groups A and B.
Patients with coronary artery disease have lower serum and pericardial fluid levels of endostatin and higher serum levels of VEGF. Serum level VEGF, but not endostatin, is associated with good or poor collateralization in patients with coronary artery disease.
背景/目的：血管内皮生长因子（VEGF）和内皮抑素与缺血性心脏病有关。本研究调查了有无缺血性心脏病患者心包液和血清中VEGF和内皮抑素的水平。
共有39例接受心脏直视手术的患者入组（24例为患有严重冠状动脉疾病的CAD组；15例为无冠状动脉疾病的非CAD组）。在CAD组中，患者根据冠状动脉侧支循环良好（A组；n = 11）或冠状动脉侧支循环不良（B组；n = 13）进行分类。手术时采集心包液和血清样本。通过酶联免疫吸附测定法测量VEGF和内皮抑素。
CAD组血清和心包液中的内皮抑素水平均显著低于非CAD组（分别为130.5±37.3 ng/mL对172.4±37.8 ng/mL和119.0±25.0 ng/mL对143.0±23.5 ng/mL）。CAD组血清VEGF浓度（92.6±18.2 pg/mL）显著高于非CAD组（75.2±22.3 pg/mL）。A组血清VEGF浓度（100.1±20.7 pg/mL）显著高于B组（84.3±12.4 pg/mL）。A组和B组之间心包液VEGF、血清和心包液内皮抑素水平无显著差异。
冠状动脉疾病患者血清和心包液中的内皮抑素水平较低，血清VEGF水平较高。血清VEGF水平而非内皮抑素水平与冠状动脉疾病患者的侧支循环良好或不良有关。
