从日本慢生根瘤菌CC705菌株的大豆根瘤类菌体中分离出的三种可溶性c型细胞色素的特性分析。

Characterization of three soluble c-type cytochromes isolated from soybean root nodule bacteroids of Bradyrhizobium japonicum strain CC705.

作者信息

Appleby C A, James P, Hennecke H

机构信息

Division of Plant Industry, C.S.I.R.O., Canberra, Australia.

出版信息

FEMS Microbiol Lett. 1991 Oct 1;67(2):137-44. doi: 10.1016/0378-1097(91)90344-a.

DOI:10.1016/0378-1097(91)90344-a

PMID:1663887

Abstract

Three soluble, low molecular mass cytochromes c (Mr 8000-15,000) were isolated and purified from soybean root nodule bacteroids of Bradyrhizobium japonicum strain CC705. On the basis of their alpha: absorbance peaks in the reduced forms, they were named cytochromes c550, c552 and c555. Cytochrome c552 reacted very fast, c555 very slowly and c550 not at all with carbon monoxide. The complete amino acid sequence (73 residues) of cytochrome c552 was established which identifies it as a monoheme, class I cytochrome c with some remote similarity to the cytochrome c6 family.

摘要

从日本慢生根瘤菌CC705菌株的大豆根瘤类菌体中分离并纯化出三种可溶性低分子量细胞色素c（分子量8000 - 15,000）。根据它们还原态的α吸收峰，将它们命名为细胞色素c550、c552和c555。细胞色素c552与一氧化碳反应非常快，c555非常慢，而c550根本不与一氧化碳反应。确定了细胞色素c552的完整氨基酸序列（73个残基），它被鉴定为一种单血红素的I类细胞色素c，与细胞色素c6家族有一些远缘相似性。