Thakare J P, Gore M M, Risbud A R, Banerjee K, Ghosh S N
National Institute of Virology, Pune.
Indian J Med Res. 1991 Sep;93:271-6.
During the Japanese encephalitis (JE) epidemic in 1988 at Gorakhpur, Uttar Pradesh, 34 cerebrospinal fluid (CSF) samples with 16 matching sera from 34 anti JEV IgM positive (confirmed JE) and 24 CSF samples with 4 matching sera from 24 anti JEV IgM negative (clinical encephalitis) patients were collected and tested for presence of JEV specific IgG by ELISA. Eighteen CSF samples and 8 matching sera from confirmed JE and 5 CSF samples and one matching serum from clinical encephalitis patients positive for JEV specific IgG were further assayed for subclass specificity using specific murine monoclonal antibodies. Almost all the samples exhibited IgG1 as the virus specific subclass. In addition to IgG1, one serum and one CSF sample each from two different confirmed JE patients showed the presence of virus specific IgG4 and IgG3 respectively. Half of the confirmed JE and clinical encephalitis patients exhibited intrathecal synthesis as evident from either elevated IgG index or CSF IgG/CSF albumin ratio. Most of the patients who recovered had predominantly virus specific IgG1 in CSF. It seems likely that IgG1 might have a protective role in clearance of virus from the central nervous system.
1988年在印度北方邦戈勒克布尔发生日本脑炎（JE）疫情期间，收集了34例抗日本脑炎病毒（JEV）IgM阳性（确诊为JE）患者的34份脑脊液（CSF）样本及16份匹配血清，以及24例抗JEV IgM阴性（临床脑炎）患者的24份CSF样本及4份匹配血清，通过酶联免疫吸附测定（ELISA）检测JEV特异性IgG的存在情况。对确诊为JE且JEV特异性IgG呈阳性的18份CSF样本及8份匹配血清，以及临床脑炎患者且JEV特异性IgG呈阳性的5份CSF样本及1份匹配血清，使用特异性鼠单克隆抗体进一步检测亚类特异性。几乎所有样本均显示IgG1为病毒特异性亚类。除IgG1外，来自两名不同确诊JE患者的一份血清和一份CSF样本分别显示存在病毒特异性IgG4和IgG3。从IgG指数升高或CSF IgG/CSF白蛋白比值可看出，一半的确诊JE患者和临床脑炎患者表现出鞘内合成。大多数康复患者的CSF中主要为病毒特异性IgG1。似乎IgG1可能在从中枢神经系统清除病毒方面具有保护作用。
