Kimaro W H, Madekurozwa M-C
Department of Veterinary Anatomy, Sokoine University of Agriculture, P.O. Box 3016, Morogoro, Tanzania.
Exp Brain Res. 2006 Aug;173(2):291-7. doi: 10.1007/s00221-006-0488-5. Epub 2006 Apr 26.
The innervation of the ovary has been studied in various species of birds and mammals. Despite the fact that the innervation of any organ is an essential factor in controlling its growth and function, no information is available on the distribution of nerve fibers in the ovary of the sexually immature ostrich. Thus, the present study was undertaken to investigate the distribution of nerve fibers in the ovary of the sexually immature ostrich, using antibodies against neurofilament protein type M of 160 kD (NP), protein gene product 9.5 (PGP 9.5) and neuron specific enolase (NSE). A total of 26 sexually immature female ostriches, aged between 12 and 14 months were used in the present study. Immunostaining was performed using a LSAB plus kit (Dakocytomation, Denmark). Antibodies against NP and PGP 9.5 were used at dilutions of 1:25 and 1:50, respectively. A ready-to-use solution containing antibodies against NSE was also used. Strong immunostaining for NP, PGP 9.5 and NSE was observed in nerve bundles, which coursed through the ovarian stalk and extended into the medulla and cortex. In addition, NSE immunoreactive nerve cell bodies were observed in the cortex and medulla. NP, PGP 9.5 and NSE immunoreactive nerve fibers were present in the thecal layer of the follicular wall. The current study has highlighted the distribution of NP, PGP 9.5 and NSE-immunoreactive nerve fibers in the ovary of the sexually immature ostrich. The findings of the present study suggest that the distribution of nerve fibers in the immature ostrich is similar to that of the domestic fowl.
人们已经在多种鸟类和哺乳动物中研究了卵巢的神经支配情况。尽管任何器官的神经支配都是控制其生长和功能的重要因素，但关于性未成熟鸵鸟卵巢中神经纤维的分布尚无相关信息。因此，本研究旨在利用针对160 kD的M型神经丝蛋白（NP）、蛋白基因产物9.5（PGP 9.5）和神经元特异性烯醇化酶（NSE）的抗体，研究性未成熟鸵鸟卵巢中神经纤维的分布。本研究共使用了26只年龄在12至14个月之间的性未成熟雌性鸵鸟。免疫染色采用LSAB plus试剂盒（丹麦达科公司）进行。抗NP和PGP 9.5的抗体分别以1:25和1:50的稀释度使用。还使用了一种即用型抗NSE抗体溶液。在穿过卵巢柄并延伸至髓质和皮质的神经束中观察到了强烈的NP、PGP 9.5和NSE免疫染色。此外，在皮质和髓质中观察到了NSE免疫反应性神经细胞体。NP、PGP 9.5和NSE免疫反应性神经纤维存在于卵泡壁的卵泡膜层中。本研究突出了性未成熟鸵鸟卵巢中NP、PGP 9.5和NSE免疫反应性神经纤维的分布。本研究结果表明，未成熟鸵鸟中神经纤维的分布与家鸡相似。
