Oda Eriko, Nakamura Yasuhiro, Yamamoto Munehiko, Kojiro Masamichi
Department of Chemistry, Kurume University School of Medicine, Kurume 830-0011, Japan.
Kurume Med J. 2005;52(4):117-25. doi: 10.2739/kurumemedj.52.117.
Tubulin is the major constituent protein of microtubules. In mammals, there are seven beta-tubulins and six alpha-tubulins. Each beta-tubulin isotype has a unique tissue distribution. The purpose of this study was to describe the distribution of tubulin beta II in normal and neoplastic human tissues with immunohistochemical techniques. We obtained normal tissues from 33 cases (8 fetuses, 17 neonates, 3 children and 5 adults) and 121 samples of neoplastic tissue from surgical specimens or at autopsy. Immunohistochemical staining for tubulin beta II was performed using a monoclonal antibody, KNY379 developed in our laboratory. Tubulin beta II was detected in various normal tissues, particularly in fetal and neonatal tissues, such as the nervous system, pulmonary alveoli, bronchioles and bronchi, colon, pancreatic ducts and acini, renal convoluted tubuli, skin epidermis, body cavity mesothelial cells, smooth muscle and thymus. In the adult, broad expression was also observed; however, the immunoreactivity was weaker and the extent of its distribution decreased with age. In neoplastic tissues, tubulin beta II immunoreactivity was detected in various nervous system neoplasms and other neoplasms such as pancreatic solid cystic carcinoma, pleomorphic adenoma, Warthin's tumor, nephroblastoma, basal cell carcinoma and malignant mesothelioma. We conclude that our monoclonal antibody, KNY379, may be useful as a marker of nervous system neoplasm, pancreatic solid cystic carcinoma, pleomorphic adenoma, Warthin's tumor, nephroblastoma, basal cell carcinoma and malignant mesothelioma.
微管蛋白是微管的主要组成蛋白。在哺乳动物中，有七种β-微管蛋白和六种α-微管蛋白。每种β-微管蛋白亚型都有独特的组织分布。本研究的目的是用免疫组织化学技术描述微管蛋白βII在正常和肿瘤性人类组织中的分布。我们从33例（8例胎儿、17例新生儿、3例儿童和5例成人）获取了正常组织，并从手术标本或尸检中获得了121份肿瘤组织样本。使用我们实验室研发的单克隆抗体KNY379对微管蛋白βII进行免疫组织化学染色。在各种正常组织中检测到微管蛋白βII，特别是在胎儿和新生儿组织中，如神经系统、肺泡、细支气管和支气管、结肠、胰管和腺泡、肾曲小管、皮肤表皮、体腔间皮细胞、平滑肌和胸腺。在成年人中也观察到广泛表达；然而，免疫反应性较弱，其分布范围随年龄增长而减少。在肿瘤组织中，在各种神经系统肿瘤和其他肿瘤如胰腺实性囊性癌、多形性腺瘤、沃辛瘤、肾母细胞瘤、基底细胞癌和恶性间皮瘤中检测到微管蛋白βII免疫反应性。我们得出结论，我们的单克隆抗体KNY379可能作为神经系统肿瘤、胰腺实性囊性癌、多形性腺瘤、沃辛瘤、肾母细胞瘤、基底细胞癌和恶性间皮瘤的标志物有用。
