Ramael M, Deblier I, Eerdekens C, Lemmens G, Jacobs W, Van Marck E
Department of Pathology, University Hospital Antwerp, Belgium.
J Pathol. 1993 Apr;169(4):421-4. doi: 10.1002/path.1711690406.
Immunohistochemical staining of 36 malignant mesotheliomas and 45 cases of non-neoplastic mesothelium including 20 specimens with signs of hyperplasia were investigated using murine monoclonal antibodies directed against p21 ras protein, Ha-ras protein, K-ras protein, and N-ras protein. All cases of non-neoplastic mesothelium and the majority of the malignant mesotheliomas (78 per cent) showed cytoplasmic and often submembranous immunoreactivity in more than 50 per cent of the cells with both the pan-ras and N-ras antibody. No immunoreactivity was observed for Ha- and K-ras. There was no statistically significant difference with respect to immunoreactivity between neoplastic and non-neoplastic mesothelium or between the various mesothelioma subtypes.
使用针对p21 ras蛋白、Ha-ras蛋白、K-ras蛋白和N-ras蛋白的鼠单克隆抗体，对36例恶性间皮瘤和45例非肿瘤性间皮（包括20例有增生迹象的标本）进行免疫组织化学染色研究。所有非肿瘤性间皮病例以及大多数恶性间皮瘤（78%）在超过50%的细胞中显示出细胞质且通常为膜下免疫反应性，同时使用泛ras和N-ras抗体。未观察到Ha-ras和K-ras的免疫反应性。肿瘤性和非肿瘤性间皮之间或各种间皮瘤亚型之间在免疫反应性方面无统计学显著差异。
