Bojanić Ines, Golubić Cepulić Branka, Nemet Damir, Rajić Ljubica, Batinić Drago, Labar Boris
Zavod za klinicku transfuziologiju, Klinicki zavod za laboratorijsku dijagnostiku, KBC Zagreb.
Lijec Vjesn. 2006 Jan-Feb;128(1-2):43-8.
Autologous peripheral blood stem cells are increasingly used for transplantation instead of bone marrow, even in small children and adolescents. We analyzed 73 autologous leukaphereses performed in 25 children (36% males, 64% females). The median age was 15 years (range 3-18) and the median body weight 48 kg (range 16-67). The apheresis procedures were carried out with cell separator COBE Spectra. Each patient underwent a median of 2 collections (range 1-6). The median total nucleated cell yield was 11.86x108/kg (range 1.94-21.21), mononuclear cell yield was 6.01x10(8)/kg (range 0.97-12.73) and CD34+ cell yield was 3.5 x10(6)/kg (range 0.19-28.01). During 6 (8.22%) procedures the patients had experienced apheresis-related side effects. The citrate-induced reactions were most commonly observed. The reactions were mild and cessation of collection was required only in one case, because of catheter related complication. Our results show that leukapheresis in pediatric patients is a safe procedure, well tolerated and with a very low risk of serious adverse events.
即使在小儿和青少年中，自体外周血干细胞也越来越多地被用于移植而非骨髓移植。我们分析了25名儿童（36%为男性，64%为女性）进行的73次自体白细胞分离术。中位年龄为15岁（范围3 - 18岁），中位体重为48千克（范围16 - 67千克）。采用COBE Spectra细胞分离仪进行白细胞分离术。每位患者平均进行2次采集（范围1 - 6次）。总核细胞产量中位数为11.86×10⁸/kg（范围1.94 - 21.21），单个核细胞产量为6.01×10⁸/kg（范围0.97 - 12.73），CD34⁺细胞产量为3.5×10⁶/kg（范围0.19 - 28.01）。在6次（8.22%）操作过程中，患者出现了与白细胞分离术相关的副作用。最常观察到的是枸橼酸盐诱导的反应。这些反应较为轻微，仅1例因导管相关并发症而需要停止采集。我们的结果表明，儿科患者的白细胞分离术是一种安全的操作，耐受性良好，严重不良事件风险极低。
