Zhang Zhongping, Gao Daming, Zhao Hui, Xie Chenggen, Guan Guijian, Wang Dapeng, Yu Shu-Hong
Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, P.R. China.
J Phys Chem B. 2006 May 4;110(17):8613-8. doi: 10.1021/jp060838u.
In this paper, we report a simple polypeptide-directed strategy for fabricating large spherical assembly of CaCO(3) nanoparticles. Stepwise growth and assembly of a large number of nanoparticles have been observed, from the formation of an amorphous liquidlike CaCO(3)-polypeptide precursor, to the crystallization and stabilization of polypeptide-capped nanoparticles, and eventually, the spherical assembly of nanoparticles. The "soft" poly(aspartate)-capping layer binding on a nanoparticle surface resulted in the unusual soft nature of nanoparticle assembly, providing a reservoir of primary nanoparticles with a moderate mobility, which is the basis of a new strategy for reconstructing nanoparticle assembly into complex nanoparticle architectures. Moreover, the findings of the secondary assembly of nanoparticle microspheres and the morphology transformation of nanoparticle assembly demonstrate a flexible and controllable pathway for manipulating the shapes and structures of nanoparticle assembly. In addition, the combination of the polypeptide with a double hydrophilic block copolymer (DHBC) allows it to possibly further control the shape and complexity of the nanoparticle assembly. A clear perspective is shown here that more complex nanoparticle materials could be created by using "soft" biological proteins or peptides as a mediating template at the organic-inorganic interface.
在本文中，我们报道了一种用于制备碳酸钙纳米粒子大型球形聚集体的简单多肽导向策略。已观察到大量纳米粒子的逐步生长和组装过程，从无定形类液态碳酸钙 - 多肽前体的形成，到多肽包覆纳米粒子的结晶和稳定化，最终形成纳米粒子的球形聚集体。结合在纳米粒子表面的“软”聚天冬氨酸包覆层导致纳米粒子聚集体具有异常的软性质，提供了具有适度流动性的初级纳米粒子库，这是将纳米粒子聚集体重构为复杂纳米粒子结构的新策略的基础。此外，纳米粒子微球的二次组装以及纳米粒子聚集体的形态转变研究结果展示了一种灵活且可控的途径来操纵纳米粒子聚集体的形状和结构。此外，多肽与双亲水嵌段共聚物（DHBC）的结合使其有可能进一步控制纳米粒子聚集体的形状和复杂性。此处展示了一个清晰的前景，即通过在有机 - 无机界面使用“软”生物蛋白质或肽作为介导模板，可以创造出更复杂的纳米粒子材料。
