Ahmad Zahoor, Sharma Sadhna, Khuller Gopal K
Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
FEMS Microbiol Lett. 2006 May;258(2):200-3. doi: 10.1111/j.1574-6968.2006.00224.x.
The aim of the present study was to evaluate the chemotherapeutic potential of econazole against latent tuberculosis. The activity of econazole and clotrimazole was tested against the latent bacilli (Mycobacterium tuberculosis H(37)Rv) developed by nutrient starvation under in vitro conditions and by drugs under in vivo conditions. The latent bacteria developed under in vitro latent conditions were acid-fast negative, nonreplicating, resistant to conventional antitubercular drugs and showed low respiration rates. Econazole as well as clotrimazole were found to have strong antimycobacterial potential against latent Mycobacterium tuberculosis under in vitro conditions as seen by reductions in colony-forming units. Further, econazole prevented the formation of drug-induced latency and significantly reduced bacterial burden from lungs and spleens of latent tuberculosis-infected mice. We conclude that azole drugs bear significant therapeutic potential against latent tuberculosis.
本研究的目的是评估益康唑对潜伏性结核的化疗潜力。在体外条件下通过营养饥饿以及在体内条件下通过药物诱导，测试了益康唑和克霉唑对潜伏性杆菌（结核分枝杆菌H(37)Rv）的活性。在体外潜伏条件下形成的潜伏细菌抗酸染色阴性、不复制、对传统抗结核药物耐药且呼吸速率较低。从集落形成单位的减少情况来看，在体外条件下，益康唑和克霉唑对潜伏性结核分枝杆菌均具有较强的抗分枝杆菌潜力。此外，益康唑可防止药物诱导的潜伏状态形成，并显著降低潜伏性结核感染小鼠肺和脾中的细菌负荷。我们得出结论，唑类药物对潜伏性结核具有显著的治疗潜力。
