Akanuma Genki, Habu Chiemi, Natori Yousuke, Murayama Rikinori, Nanamiya Hideaki, Kawamura Fujio
Laboratory of Molecular Genetics and Frontier Project Life's Adaptation Strategies to Environmental Changes, Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan.
FEMS Microbiol Lett. 2006 May;258(2):220-6. doi: 10.1111/j.1574-6968.2006.00234.x.
During development of a novel method for constructing a series of deletions in Bacillus subtilis using an isogenic set of gene-disrupted mutants created by integration of pMutin, deletion of the trnS operon, consisting of seven tRNA genes, was found to affect cell growth, development of competence and spore formation. A suppressor (sts1) of the DeltatrnS mutant was isolated, sequenced and found to have undergone a single base change, CAG to GAG, in the first anticodon of tRNA(Leu), in the trnB operon.
在开发一种利用通过整合pMutin创建的同基因基因破坏突变体系在枯草芽孢杆菌中构建一系列缺失的新方法时，发现由七个tRNA基因组成的trnS操纵子的缺失会影响细胞生长、感受态的发育和孢子形成。分离出了ΔtrnS突变体的一个抑制子（sts1），对其进行测序后发现，trnB操纵子中tRNA（Leu）的第一个反密码子发生了单个碱基变化，从CAG变为GAG。
