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枯草芽孢杆菌赭石色抑制基因sup-3位于一个由七个tRNA基因组成的操纵子中。

The Bacillus subtilis ochre suppressor sup-3 is located in an operon of seven tRNA genes.

作者信息

Garrity D B, Zahler S A

机构信息

Section of Genetics and Development, Cornell University, Ithaca, New York 14853.

出版信息

J Bacteriol. 1993 Oct;175(20):6512-7. doi: 10.1128/jb.175.20.6512-6517.1993.

DOI:10.1128/jb.175.20.6512-6517.1993
PMID:7691797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206761/
Abstract

Most Bacillus subtilis tRNA genes have been isolated from lambda libraries by use of probes that hybridize to tRNA or rRNA sequences. None of those genes map to the region of the sup-3 mutation. By cloning of the sup-3 allele, a cluster of seven tRNA genes (the trnS operon) that had not been isolated by other methods was identified. In principle, this approach could be used to isolate at least one more predicted tRNA-containing operon in this bacterium. The trnS operon was shown to contain tRNA genes for Asn (GUU), Ser (GCU), Glu (UUC), Gln (UUG), Lys (UUU), Leu (UAG), and Leu (GAG). The sup-3 mutation was found to be a T-to-A transversion that changes the anticodon of the lysine tRNA from 5'-UUU-3' to 5'-UUA-3'. This result agrees with previous work that determined that the sup-3 mutation causes lysine to be inserted at ochre nonsense mutations.

摘要

大多数枯草芽孢杆菌的tRNA基因是通过使用与tRNA或rRNA序列杂交的探针从λ文库中分离出来的。这些基因中没有一个定位于sup-3突变区域。通过克隆sup-3等位基因，鉴定出了一组七个tRNA基因（trnS操纵子），这些基因是通过其他方法未分离到的。原则上，这种方法可用于在这种细菌中至少再分离出一个预测含tRNA的操纵子。trnS操纵子被证明包含天冬酰胺（GUU）、丝氨酸（GCU）、谷氨酸（UUC）、谷氨酰胺（UUG）、赖氨酸（UUU）、亮氨酸（UAG）和亮氨酸（GAG）的tRNA基因。发现sup-3突变是一种T到A的颠换，它将赖氨酸tRNA的反密码子从5'-UUU-3'变为5'-UUA-3'。这一结果与先前的研究一致，该研究确定sup-3突变导致在赭石型无义突变处插入赖氨酸。

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本文引用的文献

1
Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.枯草芽孢杆菌中与rRNA操纵子rrnD和rrnE相关的两个tRNA基因簇。
J Bacteriol. 1993 Jan;175(2):503-9. doi: 10.1128/jb.175.2.503-509.1993.
2
Codon recognition rules in yeast mitochondria.酵母线粒体中的密码子识别规则。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3167-70. doi: 10.1073/pnas.77.6.3167.
3
Different pattern of codon recognition by mammalian mitochondrial tRNAs.哺乳动物线粒体tRNA对密码子的识别模式不同。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3164-6. doi: 10.1073/pnas.77.6.3164.
4
Defective specialized SP beta transducing bacteriophages of Bacillus subtilis that carry the sup-3 or sup-44 gene.携带sup-3或sup-44基因的枯草芽孢杆菌缺陷型特异性SPβ转导噬菌体。
J Bacteriol. 1981 Dec;148(3):1012-5. doi: 10.1128/jb.148.3.1012-1015.1981.
5
Novel features in the genetic code and codon reading patterns in Neurospora crassa mitochondria based on sequences of six mitochondrial tRNAs.基于六种线粒体tRNA序列的粗糙脉孢菌线粒体遗传密码和密码子阅读模式的新特征
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3159-63. doi: 10.1073/pnas.77.6.3159.
6
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
7
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J Biol Chem. 1984 Mar 25;259(6):3694-702.
8
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Nucleic Acids Res. 1983 Sep 24;11(18):6301-18. doi: 10.1093/nar/11.18.6301.
9
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Nucleic Acids Res. 1983 Aug 25;11(16):5763-74. doi: 10.1093/nar/11.16.5763.
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