Vancek Matús, Vidová Monika, Majerník Alan I, Smigán Peter
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic.
FEMS Microbiol Lett. 2006 May;258(2):269-73. doi: 10.1111/j.1574-6968.2006.00232.x.
The effect of Ca2+ ions on methanogenesis and growth of Methanothermobacter thermautotrophicus was investigated. The calcium chelator ethylene glycol bis(2-aminoethylether)-N,N,N',N'-tetra-acetic acid, calcium ionophore A23187 and ruthenium red all inhibited growth of this strain. Methane formation was strongly dependent on the external Ca2+ concentration in a resting cell suspension. In addition, methanogenesis of Ca2+ preloaded cells was stimulated by 400%. Inhibitor studies revealed that Co2+ and Ni2+, inorganic antagonists of Ca2+ transport, strongly inhibited methanogenesis in these cells. Interestingly, our findings imply that one of the enzymes of methanogenesis might catalyse a Ca2+ -dependent step and allow a direct activation of methanogenesis by Ca2+ ions.
研究了Ca2+离子对嗜热自养甲烷杆菌产甲烷作用和生长的影响。钙螯合剂乙二醇双（2-氨基乙醚）-N,N,N',N'-四乙酸、钙离子载体A23187和钌红均抑制该菌株的生长。在静止细胞悬液中，甲烷生成强烈依赖于外部Ca2+浓度。此外，预加载Ca2+的细胞的产甲烷作用提高了400%。抑制剂研究表明，Ca2+转运的无机拮抗剂Co2+和Ni2+强烈抑制这些细胞中的产甲烷作用。有趣的是，我们的研究结果表明，产甲烷作用的一种酶可能催化一个依赖Ca2+的步骤，并允许Ca2+离子直接激活产甲烷作用。
