Boumann Henry A, Hopmans Ellen C, van de Leemput Ingrid, Op den Camp Huub J M, van de Vossenberg Jack, Strous Marc, Jetten Mike S M, Sinninghe Damsté Jaap S, Schouten Stefan
Department of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research (NIOZ), Den Burg, The Netherlands.
FEMS Microbiol Lett. 2006 May;258(2):297-304. doi: 10.1111/j.1574-6968.2006.00233.x.
Anammox bacteria present in wastewater treatment systems and marine environments are capable of anaerobically oxidizing ammonium to dinitrogen gas. This anammox metabolism takes place in the anammoxosome which membrane is composed of lipids with peculiar staircase-like 'ladderane' hydrocarbon chains that comprise three or four linearly concatenated cyclobutane structures. Here, we applied high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to elucidate the full identity of these ladderane lipids. This revealed a wide variety of ladderane lipid species with either a phosphocholine or phosphoethanolamine polar headgroup attached to the glycerol backbone. In addition, in silico analysis of genome data gained insight into the machinery for the biosynthesis of the phosphocholine and phosphoethanolamine phospholipids in anammox bacteria.
存在于废水处理系统和海洋环境中的厌氧氨氧化细菌能够在厌氧条件下将铵氧化为氮气。这种厌氧氨氧化代谢发生在厌氧氨氧化体中，其膜由具有特殊阶梯状“梯烷”烃链的脂质组成，这些烃链包含三个或四个线性连接的环丁烷结构。在这里，我们应用高效液相色谱与电喷雾电离串联质谱以及基质辅助激光解吸/电离飞行时间质谱联用技术，以阐明这些梯烷脂质的完整特性。这揭示了多种梯烷脂质种类，其磷酸胆碱或磷酸乙醇胺极性头部基团连接在甘油主链上。此外，对基因组数据的计算机分析深入了解了厌氧氨氧化细菌中磷酸胆碱和磷酸乙醇胺磷脂的生物合成机制。
