脉冲场凝胶电泳在肠道病原菌暴发分子分型方法中的应用

[Application of pulse field gel electrophoresis in molecular typing method on dealing with the outbreak of intestinal pathogen].

作者信息

Wang Xiao-meng, Younan Alida, Takashi Masuda

机构信息

Department of Tuberculosis, Center for Disease Control and Prevention of Zhejiang Provincial, Hangzhou 310009, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Mar;40(2):109-12.

PMID:16640911

Abstract

OBJECTIVE

To explore and establish fast and sensitive typing diagnostic methods on intestinal pathogen.

METHODS

All 29 isolates of Shigella. sonnei and 12 strains of Escherichia coli O157:H7 (EHEC O157:H7) isolated in 1999 were carried out for typing by using pulse-field gel electrophoresis (PFGE) and phage typing.

RESULTS

PFGE results of 4 outbreak incidents caused by Shigella. sonnei showed that the PFGE patterns of the strains isolated from the same incident were almost the same. It was also the same during analysing the 2 outbreak incidents caused by EHEC O157:H7, however, the PFGE patterns of the 3 individual strains of EHEC O157:H7 were different. Moreover, the phage typing method showed its fine typing ability while its being used in the analysing 12 strains of EHEC O157:H7.

CONCLUSION

The fine typing ability and reliability of PFGE was helpful for finding out the heredity background of intestinal pathogen and the infectious resource of the outbreak. And the epidemic situation might thus be effectively prevented from spreading.

摘要

目的

探索并建立快速、灵敏的肠道病原菌分型诊断方法。

方法

对1999年分离出的29株宋内志贺菌和12株肠出血性大肠杆菌O157:H7（EHEC O157:H7）采用脉冲场凝胶电泳（PFGE）和噬菌体分型进行分型。

结果

由宋内志贺菌引起的4起暴发事件的PFGE结果显示，同一事件分离出的菌株PFGE图谱几乎相同。分析由EHEC O157:H7引起的2起暴发事件时情况也是如此，然而，3株单独的EHEC O157:H7菌株的PFGE图谱不同。此外，噬菌体分型方法在分析12株EHEC O157:H7时显示出良好的分型能力。