Izumiya H, Masuda T, Ahmed R, Khakhria R, Wada A, Terajima J, Itoh K, Johnson W M, Konuma H, Shinagawa K, Tamura K, Watanabe H
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
Microbiol Immunol. 1998;42(7):515-9. doi: 10.1111/j.1348-0421.1998.tb02318.x.
A total of 236 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates in Japan were investigated by bacteriophage typing, and the results were compared with those of pulsed-field gel electrophoresis (PFGE). Seven phage types (PTs) were observed in 71 isolates which were derived from 22 outbreaks. All of the isolates from ten outbreaks in the Kinki region (midwestern part of Japan) in July-August 1996 were grouped into the same PFGE type (IIa) and PT 32, while among total isolates, there were such varieties as PFGE type IIa containing five phage types and PT32 containing two PFGE types. These results suggest that the ten outbreaks should be considered to be a single outbreak, and show that the combined use of bacteriophage typing and PFGE enhances reliability in epidemiological surveys.
对日本的236株肠出血性大肠杆菌（EHEC）O157:H7分离株进行了噬菌体分型研究，并将结果与脉冲场凝胶电泳（PFGE）结果进行了比较。在源自22起疫情的71株分离株中观察到7种噬菌体类型（PTs）。1996年7 - 8月在近畿地区（日本中西部）的10起疫情中的所有分离株都被归为同一PFGE类型（IIa）和PT 32，而在所有分离株中，存在诸如包含5种噬菌体类型的PFGE IIa型和包含2种PFGE类型的PT32等多种类型。这些结果表明，这10起疫情应被视为一次单一疫情，并且表明噬菌体分型和PFGE的联合使用提高了流行病学调查的可靠性。
