[树突状细胞疫苗对裸鼠移植性舌鳞状细胞癌肿瘤生长的抑制作用]

[Inhibitory effect of dendritic cell vaccine on the growth of implanted tongue squamous cell carcinoma tumors in nude mice].

作者信息

Han Wei, Hu Qin-gang, Wang Zhi-yong

机构信息

Department of Oral and Maxillofacial Surgery, The Stomatological Hospital Affiliated to Medical college of Nanjing University, Nanjing 210008, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Feb;41(2):81-4.

PMID:16640929

Abstract

OBJECTIVE

To investigate the inhibitory effect of dendritic cells (DCs) on the growth of the implanted tongue squamous cell carcinoma (SCC) tumors in nude mice.

METHODS

Being induced from human peripheral blood monocytes (PBMCs) with rhGM-CSF, rhIL-4 and rhTNF-alpha, DCs were pulsed by Tca8113 cells lysates. Those DCs and T lymphocytes were co-cultured to induce specific cytotoxic T lymphocytes (CTLs). Immunotherapy was then performed after those DCs and CTLs were implanted into the tumor-bearing nude mice.

RESULTS

DCs were induced from PBMCs with multiple cytokines. Compared with the control groups, the growth of tumors was significantly inhibited in the group that had been implanted with DCs and CTLs, and tumor doubling time also increased markedly (P < 0.05).

CONCLUSIONS

DCs function normally after being induced from human PBMCs with multiple cytokines; DCs pulsed with tumor cell lysates and CTLs co-cultured with those DCs have a significant anti-tumor immune activity in nude mice.

摘要

目的

探讨树突状细胞（DCs）对裸鼠移植性舌鳞状细胞癌（SCC）肿瘤生长的抑制作用。

方法

用人外周血单核细胞（PBMCs）经重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）、重组人白细胞介素-4（rhIL-4）和重组人肿瘤坏死因子-α（rhTNF-α）诱导生成DCs，并用Tca8113细胞裂解物脉冲处理这些DCs。将这些DCs与T淋巴细胞共培养以诱导特异性细胞毒性T淋巴细胞（CTLs）。然后将这些DCs和CTLs植入荷瘤裸鼠体内后进行免疫治疗。