口腔浸润性癌中基质CD34和α-平滑肌肌动蛋白的表达
[Expression of stromal CD34 and alpha-smooth muscle actin in oral invasive cancer].
作者信息
Shi Lin, Sun Shan-zhen, Wang Zhen-guang
机构信息
Institute of Pathology, School of Stomatology, Shandong University, Jinan 250012, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Feb;41(2):106-7.
OBJECTIVE
To observe the expression of stromal CD34 and alpha-smooth muscle actin (alpha-SMA) in oral invasive cancer.
METHODS
The distribution and expression of stromal CD34 and alpha-SMA in 60 patients with invasive oral squamous cell cancer and 20 resections with tumor-free margins from 60 patients with invasive oral squamous cell cancer were examined by immunohistochemistry SP method.
RESULTS
Twenty cases of resections of mucosa with tumor-free margins exhibited CD34 expression in fibrocytes cytoplasm in the vicinity of vessels, mucosa and submucosa but were free of alpha-SMA expression, only the walls of muscularized vessels stained positive for alpha-SMA in the stroma. Sixty invasive oral squamous cell carcinomas were free of stromal CD34 expression, only the endothelia of small vessels stained positive for CD34, of which 53 invasive oral squamous cell carcinomas expressed stromal alpha-SMA in myofibroblasts cytoplasm.
CONCLUSIONS
The detection of CD34 and alpha-SMA is an adjunctive tool in judging oral cancer invasion in small oral mucosa biopsy specimens based on the absence of CD34 expression paralleled by the gain of alpha-SMA in the stroma of oral invasive cancer.
目的
观察基质CD34和α-平滑肌肌动蛋白（α-SMA）在口腔浸润性癌中的表达。
方法
采用免疫组织化学SP法检测60例口腔浸润性鳞状细胞癌患者及60例口腔浸润性鳞状细胞癌患者的20例切缘无瘤组织中基质CD34和α-SMA的分布及表达情况。
结果
20例切缘无瘤的黏膜切除标本中，血管、黏膜及黏膜下附近的纤维细胞质中可见CD34表达，但无α-SMA表达，仅基质中肌化血管壁α-SMA染色阳性。60例口腔浸润性鳞状细胞癌基质中无CD34表达，仅小血管内皮CD34染色阳性，其中53例口腔浸润性鳞状细胞癌肌成纤维细胞质中表达基质α-SMA。
结论
基于口腔浸润性癌基质中CD34表达缺失而α-SMA表达增加，检测CD34和α-SMA是判断小口腔黏膜活检标本中口腔癌浸润的辅助工具。
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