Scheithauer R, Weisser H J
Institut für Rechtsmedizin, Albert-Ludwig-Universität Freiburg, Federal Republic of Germany.
Int J Legal Med. 1991;104(5):273-5. doi: 10.1007/BF01369584.
Linen was pretreated with 20 remedies for cleaning and maintaining clothes and 20 microliters blood was applied on each sample and dried. Restriction enzyme digest of bloodstain DNA was irregularly inhibited by highly concentrated residues of 2 detergents and a stain-remover and colour-brightener. An additional dialysis step to purify DNA (Gill 1987) reliably prevented disturbance. High molecular DNA was obtained in every case and bandshifts were not observed.
亚麻布用20种清洗和保养衣物的药剂进行预处理,然后在每个样本上滴加20微升血液并干燥。两种洗涤剂以及一种去污增白剂的高浓度残留会不规则地抑制血迹DNA的限制性内切酶消化。通过额外的透析步骤来纯化DNA(吉尔,1987年)可有效防止干扰。在每种情况下均获得了高分子量DNA,且未观察到条带迁移。