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对用衣物清洁保养用药物预处理过的亚麻布上的血迹进行DNA分析。

DNA profiling of bloodstains on linen pretreated with remedies used for cleaning and maintaining clothes.

作者信息

Scheithauer R, Weisser H J

机构信息

Institut für Rechtsmedizin, Albert-Ludwig-Universität Freiburg, Federal Republic of Germany.

出版信息

Int J Legal Med. 1991;104(5):273-5. doi: 10.1007/BF01369584.

DOI:10.1007/BF01369584
PMID:1664233
Abstract

Linen was pretreated with 20 remedies for cleaning and maintaining clothes and 20 microliters blood was applied on each sample and dried. Restriction enzyme digest of bloodstain DNA was irregularly inhibited by highly concentrated residues of 2 detergents and a stain-remover and colour-brightener. An additional dialysis step to purify DNA (Gill 1987) reliably prevented disturbance. High molecular DNA was obtained in every case and bandshifts were not observed.

摘要

亚麻布用20种清洗和保养衣物的药剂进行预处理,然后在每个样本上滴加20微升血液并干燥。两种洗涤剂以及一种去污增白剂的高浓度残留会不规则地抑制血迹DNA的限制性内切酶消化。通过额外的透析步骤来纯化DNA(吉尔,1987年)可有效防止干扰。在每种情况下均获得了高分子量DNA,且未观察到条带迁移。

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DNA profiling of bloodstains on linen pretreated with remedies used for cleaning and maintaining clothes.对用衣物清洁保养用药物预处理过的亚麻布上的血迹进行DNA分析。
Int J Legal Med. 1991;104(5):273-5. doi: 10.1007/BF01369584.
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本文引用的文献

1
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Beitr Gerichtl Med. 1988;46:193-8.
2
Postmortem stability of DNA.DNA的死后稳定性
Forensic Sci Int. 1988 Oct;39(1):59-70. doi: 10.1016/0379-0738(88)90118-1.
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Arch Kriminol. 1990 Jul-Aug;186(1-2):43-50.
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[Sperm detection using monoclonal antibodies].[使用单克隆抗体检测精子]
Beitr Gerichtl Med. 1990;48:17-20.
5
The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains.各种染色载体对从干血斑中提取的DNA质量和数量的影响。
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