Scheithauer R, Schilling K
Institut für Rechtsmedizin der Albert-Ludwigs-Universität Freiburg.
Arch Kriminol. 1990 Jul-Aug;186(1-2):43-50.
Linen has been treated with 20 different remedies for clothes (impregnating agents, fabric softeners, detergents, finishes, and stainremovers; see tab. 2) in "normal" and "high" concentration. After short, intentionally incomplete washing and after successive drying 5 microliters and 10 microliters blood each of the six major ABH types have been applied. Stains have been ABH typed by the absorption-inhibition test according to Holzer, the absorption-elution test using stain extracts according to Chisum, and another absorptions-elution test performed in tubes. Only 3 of the 20 remedies had no effect on the results (tab.3). The AI-test showed no false results, but partly reduced absorption and haemolysis of the added red blood cells. Both AE-tests gave false-positive and false-negative results. Compared with the tube test the method described by Chisum was more reliable. The rate of false results depended on the concentration of the remedies used for the treatment of the linen. The majority of the incorrect results (but not all!) could have been recognized by processing controls analogously (see tab. 4 and 5; legend in English under tab. 5).
亚麻布用20种不同的衣物处理剂（浸渍剂、织物柔软剂、洗涤剂、整理剂和去污剂；见表2）以“正常”和“高”浓度进行处理。经过短暂的、故意不完全的洗涤以及连续干燥后，分别滴加了六种主要ABH血型的5微升和10微升血液。污渍通过根据霍尔泽方法的吸收抑制试验、根据奇泽姆方法使用污渍提取物的吸收洗脱试验以及在试管中进行的另一种吸收洗脱试验进行ABH血型鉴定。20种处理剂中只有3种对结果没有影响（表3）。吸收抑制试验未出现假结果，但部分降低了添加红细胞的吸收和溶血。两种吸收洗脱试验均出现了假阳性和假阴性结果。与试管试验相比，奇泽姆描述的方法更可靠。假结果的发生率取决于用于处理亚麻布的处理剂浓度。大多数错误结果（但不是全部！）可以通过类似地处理对照来识别（见表4和5；表5下方的英文说明）。
