Hoshino Mai, Shoji Jun, Inada Noriko, Sawa Mitsuru, Kato Hiroshi
Department of Ophthalmology, Nihon University School of Medicine, Tokyo 173 8610, Japan.
Nippon Ganka Gakkai Zasshi. 2006 Apr;110(4):276-81.
To evaluate the efficacy of measurement of secretory IgA (sIgA) in tears with tear sampling methods using filter paper, and to review sIgA measurement method for clinical application.
Subjects were divided into the following 4 groups: a healthy control group, 29 eyes of 29 subjects; a contact lens group, 15 eyes of 15 subjects; a dry eye group, 13 eyes of 13 subjects; and a herpes group, 6 eyes of 6 subjects. In all subjects the sIgA value in tears was measured. In addition, in eighteen eyes of 18 healthy control individuals, the tear sIgA value was measured three times, morning, noon, and night in one day, and the variation of tear sIgA value was checked. The tears were sampled by the Schirmer 1 method. Schirmer papers were eluted in 200 microl of 0.5 M NaCl and 0.5% Tween 20 in 0.05 M phosphate-buffered solution (pH 7.2). Tear sIgA value was measured by ELISA (enzyme-linked immunosorbent assay).
Tear sIgA value was 1249.0+/-1025.0 microg/ ml in the healthy control group; 1057.4+/-1583.3 microg /ml in the contact len groups; 197.8+/-91.3 microg/ml in the dry eye group; and 759.7+/-467.8 microg/ml in the herpes group. There was no significant difference between the healthy control group and the contact lens group, or the control group and the herpes group. There was a significantly (p<0.0001) low value in the dry eye group in comparison with the healthy control group. In the 18 healthy control individuals, there was a tendency for the tear sIgA value collected at noon to be higher.
Measurment of the changes in tear sIgA values caused by inflammation of the lacrimal gland is useful as a clinical test of lacrimal gland function.
评估使用滤纸泪液采样方法测量泪液中分泌型免疫球蛋白A（sIgA）的有效性，并回顾sIgA测量方法在临床中的应用。
将受试者分为以下4组：健康对照组，29名受试者的29只眼；隐形眼镜组，15名受试者的15只眼；干眼组，13名受试者的13只眼；疱疹组，6名受试者的6只眼。测量所有受试者泪液中的sIgA值。此外，在18名健康对照个体的18只眼中，于一天中的早晨、中午和晚上分别测量泪液sIgA值3次，检查泪液sIgA值的变化情况。采用Schirmer 1法采集泪液。将Schirmer试纸在200微升含0.5%吐温20的0.5M氯化钠及0.05M磷酸盐缓冲溶液（pH 7.2）中洗脱。采用酶联免疫吸附测定法（ELISA）测量泪液sIgA值。
健康对照组泪液sIgA值为1249.0±1025.0微克/毫升；隐形眼镜组为1057.4±1583.3微克/毫升；干眼组为197.8±91.3微克/毫升；疱疹组为759.7±467.8微克/毫升。健康对照组与隐形眼镜组之间、对照组与疱疹组之间无显著差异。与健康对照组相比，干眼组的值显著较低（p<0.0001）。在18名健康对照个体中，中午采集的泪液sIgA值有较高的趋势。
测量泪腺炎症引起的泪液sIgA值变化，作为泪腺功能的临床检测是有用的。
