长期佩戴软性隐形眼镜者和非隐形眼镜佩戴者基础泪液中的分泌型IgA表位。
Secretory IgA epitopes in basal tears of extended-wear soft contact lens wearers and in non-lens wearers.
作者信息
Pearce D J, Demirci G, Willcox M D
机构信息
The Cooperative Research Centre for Eye Research and Technology, Randwick, Sydney, New South Wales, Australia.
出版信息
Aust N Z J Ophthalmol. 1999 Jun-Aug;27(3-4):221-3. doi: 10.1046/j.1440-1606.1999.00206.x.
PURPOSE
To determine secretory IgA epitopes in tears of extended-wear soft contact lens wearers and non-wearing controls.
METHODS
We developed enzyme-linked immunosorbent assays (ELISA) to determine the tear concentrations of two epitopes of secretory IgA, the IgA alpha-chain and the secretory component. These epitopes were measured in basal tears of 20 individuals in 6 nights of extended wear of etafilcon A soft contact lenses and in 19 non-lens-wearing individuals.
RESULTS
Levels of IgA alpha-chain immunoreactivity were significantly decreased in the lens-wearing group compared to non-lens wearers. However, the level of secretory component immunoreactivity was not significantly different between groups. IgA alpha-chain and secretory component immunoreactvity were highly correlated; however, some samples showed a marked variation between these two values.
CONCLUSION
Tear concentrations of sIgA epitopes are significantly reduced in extended-wear contact lens wearers, and may contribute to the increased susceptibility to ocular infection seen in this group.
目的
确定长期佩戴软性隐形眼镜者与非佩戴者泪液中的分泌型IgA表位。
方法
我们开发了酶联免疫吸附测定法（ELISA）来测定分泌型IgA的两个表位（IgAα链和分泌成分）的泪液浓度。在20名佩戴依他氟康A软性隐形眼镜6晚的个体以及19名未佩戴隐形眼镜的个体的基础泪液中测量了这些表位。
结果
与未佩戴隐形眼镜者相比，佩戴隐形眼镜组的IgAα链免疫反应性水平显著降低。然而，两组之间分泌成分免疫反应性水平无显著差异。IgAα链和分泌成分免疫反应性高度相关；然而，一些样本在这两个值之间显示出明显差异。
结论
长期佩戴隐形眼镜者泪液中sIgA表位浓度显著降低，这可能导致该组人群眼部感染易感性增加。
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