使用高效液相色谱/微量滴定板分析进行生物活性谱分析：应用于一种源自新西兰海洋藻类的真菌，粘帚霉属菌种

Bioactivity profiling using HPLC/microtiter-plate analysis: application to a New Zealand marine alga-derived fungus, Gliocladium sp.

作者信息

Lang Gerhard, Mitova Maya I, Ellis Gill, van der Sar Sonia, Phipps Richard K, Blunt John W, Cummings Nicholas J, Cole Anthony L J, Munro Murray H G

机构信息

Department of Chemistry and School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.

出版信息

J Nat Prod. 2006 Apr;69(4):621-4. doi: 10.1021/np0504917.

DOI:10.1021/np0504917

PMID:16643039

Abstract

Using HPLC/microtiter-plate-based generation of activity profiles the extract of a marine alga-derived fungus, identified as Gliocladium sp., was shown to contain the known strongly cytotoxic metabolite 4-keto-clonostachydiol (1) and also clonostachydiol (2) as well as gliotide (3), a new cyclodepsipeptide containing several D-amino acids. The absolute configuration of 1 was elucidated by reduction to 2, and two further oxidized derivatives of clonostachydiol (5, 6) were prepared and evaluated for biological activity.

摘要

利用基于高效液相色谱/微量滴定板的活性谱生成方法，一种被鉴定为粘帚霉属的海洋藻类衍生真菌的提取物被证明含有已知的强细胞毒性代谢物4-酮基棒曲霉素（1）、棒曲霉素（2）以及环缩肽gliotide（3），后者是一种含有多个D-氨基酸的新型环缩肽。通过还原为2阐明了1的绝对构型，并制备了棒曲霉素的另外两种氧化衍生物（5、6）并评估其生物活性。