Huang Jun, Ma Rui, Yang Pei-zeng, Wu Chang-you
Department of Immunology, Medical College, Sun Yat-sen University, Guangzhou 510080, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 May;22(3):346-9.
To explore the effect of cyclosporine A (CsA) on the production of IFN-gamma by murine splenocytes after stimulation with different reagents.
Mouse splenocytes were prepared and cultured with anti-CD3 mAb alone, anti-CD3 mAb plus anti-CD28 mAb or anti-CD3 mAb plus IL-12, in the presence or absence of different concentrations of CsA. After stimulating for 3 days, the content of INF-gamma in the culture supernatant was assayed by ELISA. The effects of CsA on the expression of activated molecules on T cells and intracellular IFN-gamma production were examined at the single cell level by flow cytometry.
The mouse splenocytes stimulated with anti-CD3 mAb, anti-CD3 mAb + anti-CD28 mAb or anti-CD3 mAb + IL-12 could produce high level of IFN-gamma, but CsA inhibited INF-gamma production in a dose-dependent manner. The inhibitory mechanism of CsA was related to the activation of splenocytes.
CsA can inhibit the expressions of CD25 and CD69 on splenocytes, as well as INF-gamma production by splenocytes in a dose dependent manner.
探讨环孢素A（CsA）对不同试剂刺激后小鼠脾细胞产生γ干扰素（IFN-γ）的影响。
制备小鼠脾细胞，在有或无不同浓度CsA存在的情况下，分别用抗CD3单克隆抗体（mAb）单独培养、抗CD3 mAb加抗CD28 mAb或抗CD3 mAb加白细胞介素-12（IL-12）进行培养。刺激3天后，通过酶联免疫吸附测定法（ELISA）检测培养上清液中IFN-γ的含量。通过流式细胞术在单细胞水平检测CsA对T细胞上活化分子表达及细胞内IFN-γ产生的影响。
用抗CD3 mAb、抗CD3 mAb +抗CD28 mAb或抗CD3 mAb + IL-12刺激的小鼠脾细胞可产生高水平的IFN-γ，但CsA以剂量依赖方式抑制IFN-γ的产生。CsA的抑制机制与脾细胞的活化有关。
CsA可剂量依赖性地抑制脾细胞上CD25和CD69的表达以及脾细胞产生IFN-γ。
