在十六烷基三甲基溴化铵存在下核酸对钇（III）-芦丁荧光的增强作用
Fluorescence enhancement of yttrium(III)-rutin by nucleic acids in the presence of cetyltrimethylammonium bromide.
作者信息
Wu Xia, Guo Changying, Wang Fei, Yang Jinghe, Ran Dehuan, Zheng Jinhua, Wu Jinbo
机构信息
Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2006 Nov;65(3-4):543-8. doi: 10.1016/j.saa.2005.12.009. Epub 2006 Apr 27.
It is found that nucleic acids can enhance the fluorescence intensity of yttrium(III) (Y(3+))-rutin in presence of cetyltrimethylammonium bromide (CTMAB) system. In hexamethylenetetramine (HMTA)-HCl buffer, the maximum enhanced fluorescence is produced, with maximum excitation and emission wavelengths at 452 and 520 nm, respectively. Based on this, a new fluorimetric method of determination of nucleic acids is proposed. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 1.0 x 10(-7) to 1.0 x 10(-5)g/ml for fish sperm DNA (fsDNA), 1.0 x 10(-7) to 4.6 x 10(-6)g/ml for yeast RNA (yRNA), their detection limits (S/N=3) are 7.5 x 10(-8), 8.0 x 10(-8)g/ml, respectively. The interaction mechanism is also studied.
研究发现，在十六烷基三甲基溴化铵（CTMAB）体系中，核酸能够增强钇（III）（Y(3+)）-芦丁的荧光强度。在六亚甲基四胺（HMTA）-盐酸缓冲液中，产生最大增强荧光，其最大激发波长和发射波长分别为452nm和520nm。基于此，提出了一种测定核酸的新荧光分析法。在最佳条件下，对于鱼精DNA（fsDNA），增强荧光强度在1.0×10(-7)至1.0×10(-5)g/ml范围内与核酸浓度成正比；对于酵母RNA（yRNA），在1.0×10(-7)至4.6×10(-6)g/ml范围内与核酸浓度成正比，它们的检测限（S/N = 3）分别为7.5×10(-8)、8.0×10(-8)g/ml。同时还研究了相互作用机理。
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