Hoffman Donald R
Department of Pathology and Laboratory Medicine, Brody School of Medicine at East Carolina University, Greenville, NC, USA.
Clin Rev Allergy Immunol. 2006 Apr;30(2):109-28. doi: 10.1385/criai:30:2:109.
Hymenoptera venoms each contain a variety of protein allergens. The major components have all been characterized, and most of the amino acid sequences are known. This article concentrates on the use of contemporary techniques including cloning, mass spectrometry and genomics in the characterization of venom allergens, and newer separation techniques for protein isolation. Examples of the use of these techniques with venom proteins are presented.
膜翅目昆虫毒液均含有多种蛋白质过敏原。其主要成分均已得到鉴定,且大多数氨基酸序列也已明确。本文重点介绍当代技术,包括克隆、质谱分析和基因组学在毒液过敏原鉴定中的应用,以及用于蛋白质分离的更新的分离技术。文中列举了这些技术在毒液蛋白应用中的实例。