巨片形吸虫排泄分泌抗原对大鼠血液学指标的影响。
Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices.
作者信息
Ganga G, Varshney J P, Sharma R L
机构信息
Clinical Medicine Laboratory, Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122 (U.P.), India.
出版信息
J Vet Sci. 2006 Jun;7(2):123-5. doi: 10.4142/jvs.2006.7.2.123.
The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37 degrees C, and centrifuging the supernatant at 12,000 g at 4 degrees C for 30 min. The protein content of Fg-ESA was adjusted to 1.8 mg/ml. The rats were randomly divided into two groups of six rats each. Rats in group A received 0.5 ml of Fg-ESA intraperitoneally (i.p.) for 7 days, whereas control rats in group B received 0.5 ml of PBS i.p. for 7 days. Hemograms of both groups were studied initially and on days 0, 2, 4, 14 and 21 after the final injection of Fg-ESA or PBS. Progressive and significant (p < 0.01) declines in the values of hemoglobin, hematocrit, and total erythrocyte count were observed without significant (p > 0.05) changes in the values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, or mean corpuscular volume in group A. Thus, we conclude that Fg-ESA induces normocytic normochromic anemia in rats.
本研究旨在探讨巨片形吸虫排泄分泌抗原（Fg-ESA）对大鼠血液学指标的影响。通过将40条彻底清洗后的巨片形吸虫置于100 ml磷酸盐缓冲盐水（PBS）中，在37℃下孵育2小时，然后在4℃下以12,000 g离心30分钟，制备Fg-ESA。将Fg-ESA的蛋白质含量调整至1.8 mg/ml。将大鼠随机分为两组，每组6只。A组大鼠腹腔注射（i.p.）0.5 ml Fg-ESA，共7天，而B组作为对照大鼠腹腔注射0.5 ml PBS，共7天。在首次注射Fg-ESA或PBS之前以及最后一次注射后的第0、2、4、14和21天，对两组大鼠进行血常规检查。观察到A组大鼠的血红蛋白、血细胞比容和红细胞总数的值呈进行性且显著（p < 0.01）下降，而平均红细胞血红蛋白、平均红细胞血红蛋白浓度或平均红细胞体积的值无显著（p > 0.05）变化。因此，我们得出结论，Fg-ESA可诱导大鼠发生正细胞正色素性贫血。
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