Molina Elizabeth C
School of Tropical Veterinary Science, James Cook University, Townsville, Queensland 4811, Australia.
J Vet Sci. 2005 Jun;6(2):135-9.
This study investigated the presence of cytokines interferon (IFN)-gamma, interleukins (IL) -6 and -8 in serum of cattle and buffaloes infected with Fasciola gigantica from one to 16 weeks post-infection to determine their T cell response during infection. The concentration of these cytokines was determined by sandwich enzyme-linked immunosorbent assay (ELISA). No IFN-gamma was detected in these animals while IL-6 was elevated from one to 16 weeks postinfection. Levels of IL-8 were also elevated in infected buffaloes from one to 16 weeks post-infection. A predominantly T helper (Th) 2 response which started early in the infection was apparently present in cattle and buffaloes in this study which was characterised by IL-6. IL-8 production could be another mechanism of immune response in buffaloes during infection with F. gigantica.
本研究调查了感染巨片形吸虫1至16周后牛和水牛血清中细胞因子干扰素（IFN）-γ、白细胞介素（IL）-6和-8的存在情况，以确定它们在感染期间的T细胞反应。这些细胞因子的浓度通过夹心酶联免疫吸附测定（ELISA）来确定。在这些动物中未检测到IFN-γ，而IL-6在感染后1至16周升高。感染的水牛在感染后1至16周IL-8水平也升高。本研究中牛和水牛显然存在主要由IL-6表征的、在感染早期开始的主要T辅助（Th）2反应。IL-8的产生可能是水牛在感染巨片形吸虫期间免疫反应的另一种机制。
