印度结核分枝杆菌菌株的随机扩增多态性DNA（RAPD）分析
Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India.
作者信息
Singh J P, Verma Rishendra, Chaudhuri P
机构信息
Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar-243122, U.P., India.
出版信息
J Vet Sci. 2006 Jun;7(2):181-7. doi: 10.4142/jvs.2006.7.2.181.
The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates, and it was found to be a valuable alternative epidemiological tool. In addition, the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied.
研究了随机扩增多态性DNA（RAPD）分析对印度结核分枝杆菌菌株分型的实用性。使用7种随机十聚体引物对结核分枝杆菌H37Rv、结核分枝杆菌DT和42株结核分枝杆菌临床分离株进行RAPD-PCR。发现所有7种引物都具有区分能力，并产生了特异性的RAPD图谱。多态性扩增子用作结核分枝杆菌的RAPD标记。由不同引物获得的树状图显示了引物的鉴别能力。RAPD分析提供了一种快速简便的方法来鉴定结核分枝杆菌分离株中的多态性，并且发现它是一种有价值的替代流行病学工具。此外，本研究结果显示在所研究人群中的结核分枝杆菌菌株存在异质性。
Zotero 插件