Colton Leah, Nasci Roger S
Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, PO 2087, Fort Collins, CO 80522, USA.
J Am Mosq Control Assoc. 2006 Mar;22(1):57-63. doi: 10.2987/8756-971X(2006)22[57:QOWNVI]2.0.CO;2.
Culex quinquefasciatus, Cx. restuans, Cx. pipiens complex, and Cx. nigripalpus were collected as larvae or egg rafts from the southern USA. Adult female mosquitoes were intrathoracically inoculated with approximately 1,000 plaque-forming units of West Nile virus (WNV) and saliva was collected from them 5 days later. The amount of infectious WNV in the saliva samples was quantified by plaque assay and WNV RNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). More than 90% of the mosquitoes had either infectious virus or viral RNA in their saliva. The RT-PCR assay detected a greater percent of samples with WNV RNA than the plaque assay detected infectious virus. Pairwise comparisons revealed 6 significant differences between the 7 groups surveyed. The Cx. nigripalpus secreted lower mean amounts of WNV than 3 other groups, and a difference was found between early- and late-season Cx. quinquefasciatus collected in Louisiana.
致倦库蚊、雷氏库蚊、尖音库蚊复合组以及黑须库蚊的幼虫或卵块采自美国南部。成年雌性蚊子经胸腔接种约1000个西尼罗河病毒（WNV）蚀斑形成单位，5天后收集其唾液。通过蚀斑试验对唾液样本中传染性WNV的量进行定量，并通过逆转录聚合酶链反应（RT-PCR）检测WNV RNA。超过90%的蚊子唾液中含有传染性病毒或病毒RNA。RT-PCR检测出含有WNV RNA的样本百分比高于蚀斑试验检测出传染性病毒的百分比。成对比较显示，在所调查的7组之间存在6个显著差异。黑须库蚊分泌的WNV平均量低于其他3组，并且在路易斯安那州采集的早季和晚季致倦库蚊之间也发现了差异。
