Infections of Mosquito Cells by Strains Au and Melpop Modulate Host Cellular Transcriptomes Differently and Suppress Dengue Viral Replication.

Dengue virus serotypes 1-4 (DENV1-4) have spread through tropical and subtropical countries, causing endemic and epidemic diseases. Recently, a novel field approach using the symbiont was proposed to suppress DENV transmission via the mosquito vectors and . Previously, we showed that a strain, MelPop, suppresses DENV2 replication in the C6/36 cell line, with the mutant DENV2 appearing and replacing the wild type DENV2. In this study, we expanded the analysis to include replications of all DENV serotypes 1-4, effects of Au in C6/36 cells, and MelPop-influences on the Aag2 cell line. It was revealed that both Au MelPop reduce all DENV infectious titers without dominant appearances of the mutant viruses, despite varied effects on the viral copy numbers. The host transcriptomic profiles by RNA-seq were also variously altered by Au and MelPop (ranging from 10 to 30%, LogFC > 2 or <-2, < 0.05). Those transcripts were not further altered by DENV infection. In contrast, abundant transcriptomic alterations by DENV infection in naïve C6/36 and Aag2 cells were blocked by either Au MelPop. These results indicate that prevents host cellular transcriptomic alterations which are induced by DENV infection, affecting the cellular homeostasis necessary for DENV replication.