Wheeler J G, Osorio F A
Department of Veterinary Sciences, University of Nebraska-Lincoln 68586-0905.
Am J Vet Res. 1991 Nov;52(11):1799-803.
Pseudorabies virus (PRV) latency was investigated, using polymerase chain reaction (PCR). A PCR protocol was developed that specifically amplified a 217-base pair sequence within the gene encoding the essential glycoprotein gp50 of PRV. Using this PCR procedure, the gp50 sequence was amplified from tissues of pigs infected with various doses of PRV (Becker strain). At postinoculation day 64, viral isolation was performed on nasal swab specimens and homogenates of tonsils and trigeminal nerve ganglia obtained from 11 PRV-convalescent, seropositive pigs. Results were negative in all cases. By use of PCR, 11 of 11 pigs had PRV-positive trigeminal nerve ganglia and brain stem, 10 of 11 pigs had PRV-positive tonsils, and 9 of 11 pigs had PRV-positive olfactory bulbs.
采用聚合酶链反应（PCR）对伪狂犬病病毒（PRV）潜伏感染进行了研究。开发了一种PCR方法，可特异性扩增PRV必需糖蛋白gp50编码基因内的一段217个碱基对的序列。利用该PCR程序，从感染不同剂量PRV（贝克尔株）的猪组织中扩增出gp50序列。在接种后第64天，对11头PRV康复、血清学阳性猪的鼻拭子标本以及扁桃体和三叉神经节匀浆进行病毒分离。所有病例结果均为阴性。通过PCR检测，11头猪中有11头的三叉神经节和脑干呈PRV阳性，11头猪中有10头的扁桃体呈PRV阳性，11头猪中有9头的嗅球呈PRV阳性。
