利用聚合酶链反应（PCR）检测动物病原体。
Detection of animal pathogens by using the polymerase chain reaction (PCR).
作者信息
Rodriguez J M
机构信息
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
出版信息
Vet J. 1997 May;153(3):287-305. doi: 10.1016/s1090-0233(97)80063-9.
Abstract
The polymerase chain reaction (PCR) is a nucleic acid-based technique that enables the rapid and sensitive detection of specific micro-organisms. Although this technique is widely used in veterinary research, it has not yet found applications in routine microbiological analysis of veterinary clinical samples. However, advances in sample preparation together with the increasing availability of specific gene sequences will probably lead to the more widespread diagnostic use of PCR in the future. PCR is likely to have a strong impact in the epidemiology, treatment and prevention of animal infectious diseases.
摘要
聚合酶链反应（PCR）是一种基于核酸的技术，能够快速、灵敏地检测特定微生物。尽管该技术在兽医研究中广泛应用，但尚未在兽医临床样本的常规微生物分析中得到应用。然而，样本制备技术的进步以及特定基因序列的日益可得，可能会导致未来PCR在诊断中的更广泛应用。PCR可能会对动物传染病的流行病学、治疗和预防产生重大影响。
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