Takeuchi Y, Takashima M, Katoh Y, Nishikawa T, Takahashi K
Division of Mental Disorder Research, National Center of Neurology and Psychiatry, Tokyo, Japan.
Brain Res. 1991 Nov 1;563(1-2):127-31. doi: 10.1016/0006-8993(91)91524-5.
In order to clarify the neuronal transmission mechanism of photic stimulation in the suprachiasmatic nucleus (SCN), the effects of agonists and antagonists for excitatory amino acid receptors on N-acetyltransferase (NAT) activity in the pineal gland were observed following the microinjection of drugs into both sides of the nuclei. N-Methyl-D-aspartate (NMDA), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate, and kainate (which are selective agonists for three different subtypes, i.e. NMDA, quisqualate and kainate receptors, respectively) significantly decreased NAT activity similarly to the suppressive effect of light. Moreover, compared with a control group, all the groups pretreated with a selective competitive antagonist for NMDA receptor (D-2-amino-5-phosphonovalerate or 3-((+-)-2-carboxypiperazine-4-yl)-propyl-1-phosphonate), or a selective non-competitive antagonist for non-NMDA receptors (Joro spider toxin-3 or 1-naphthylacetyl spermine) partially blocked the suppressive effect of photic stimulation on NAT activity. These results suggest that NMDA, quisqualate and kainate receptors are all involved in mediating photic stimulation in the SCN.
为了阐明视交叉上核（SCN）中光刺激的神经元传递机制，在向双侧核内微量注射药物后，观察了兴奋性氨基酸受体激动剂和拮抗剂对松果体中N - 乙酰基转移酶（NAT）活性的影响。N - 甲基 - D - 天冬氨酸（NMDA）、α - 氨基 - 3 - 羟基 - 5 - 甲基 - 4 - 异恶唑丙酸酯和海人藻酸（分别是三种不同亚型，即NMDA、quisqualate和海人藻酸受体的选择性激动剂）显著降低NAT活性，其作用与光的抑制作用相似。此外，与对照组相比，所有用NMDA受体选择性竞争性拮抗剂（D - 2 - 氨基 - 5 - 膦酸戊酯或3 - （（±） - 2 - 羧基哌嗪 - 4 - 基） - 丙基 - 1 - 膦酸酯）或非NMDA受体选择性非竞争性拮抗剂（乔罗蜘蛛毒素 - 3或1 - 萘乙酰精胺）预处理的组，部分阻断了光刺激对NAT活性的抑制作用。这些结果表明，NMDA、quisqualate和海人藻酸受体均参与介导SCN中的光刺激。
