Ito Tomoko, Iida-Tanaka Naoko, Niidome Takuro, Kawano Takahito, Kubo Koji, Yoshikawa Kenichi, Sato Toshinori, Yang Zhihong, Koyama Yoshiyuki
Department of Textile Science, Otsuma Women's University, Tokyo, Japan.
J Control Release. 2006 May 30;112(3):382-8. doi: 10.1016/j.jconrel.2006.03.013. Epub 2006 Mar 27.
Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells.
透明质酸（HA）是一种天然阴离子粘多糖，可沉积在DNA/聚乙烯亚胺（PEI）复合物的阳离子表面，以恢复表面电位并减少与蛋白质的非特异性相互作用。HA还可用作靶向特定细胞受体的配体。此外，HA包被增强了质粒/PEI复合物的转录活性，这可能是通过 loosening DNA与PEI之间的紧密结合来实现的，从而促进了转录因子的接近。具有与HMG蛋白结构相似的精胺侧链（Spn-HA）的两性HA衍生物显示出比HA更高的转录增强活性。在CHO细胞中，质粒/PEI/Spn-HA三元复合物的转基因表达效率比裸质粒/PEI复合物高29倍。 （注：原文中“loosening”未翻译完整，可能是原文有误，推测应为“loosen”，完整译文为“此外，HA包被增强了质粒/PEI复合物的转录活性，这可能是通过松开DNA与PEI之间的紧密结合来实现的，从而促进了转录因子的接近。” ）
