Shih Y L, Xu Y F, Wang W P
Shanghai Institute of Physiology, Academia Sinica.
Sheng Li Xue Bao. 1991 Jun;43(3):286-90.
By means of the intracellular recording technique, the effect of aureofuscin (20 micrograms/ml, oversaturation solution) on the ACh release from motor nerve terminals and on muscle cell membrane potential were investigated in phrenic nerve diaphragm preparations of the mice. The results showed that (a) aureofuscin reduced the resting membrane potential of the muscle cell slightly; (b) the frequency of miniature end-plate potentials and the mean quantal content of end-plate potentials increased at first and then recovered approximately to the control level; (c) the depolarization produced by aureofuscin in the muscle cell membrane was reversible and the aureofuscin-invoked facilitation in miniature end-plate potential discharges was Ca(2+)-dependent; and (d) aureofuscin did not block neuromuscular transmission.
采用细胞内记录技术，在小鼠膈神经膈肌标本中研究了金褐霉素（20微克/毫升，过饱和溶液）对运动神经末梢乙酰胆碱释放及肌细胞膜电位的影响。结果表明：（a）金褐霉素使肌细胞静息膜电位略有降低；（b）微小终板电位频率和终板电位的平均量子含量先增加，然后大致恢复到对照水平；（c）金褐霉素在肌细胞膜上产生的去极化是可逆的，且金褐霉素引起的微小终板电位发放的易化作用是钙依赖性的；（d）金褐霉素不阻断神经肌肉传递。
