Camilleri P, Okafo G N, Southan C, Brown R
SmithKline Beecham Pharmaceuticals, Welwyn, Herts, United Kingdom.
Anal Biochem. 1991 Oct;198(1):36-42. doi: 10.1016/0003-2697(91)90502-k.
The capillary electrophoresis (CE) of peptide fragments from the tryptic digest of salmon calcitonin and elcatonin has been carried out in H2O- and D2O-based buffer solutions. Analysis in heavy water was found to be superior to that in H2O especially at a pH or pD of 7.93. From a single CE run on elcatonin digest we were also able to harvest three pure cleavage peptides in sufficient quantity to determine each amino acid residue by protein sequencing. The order of elution from CE agreed with that predicted on the basis of net charge calculated for each peptide.
已在基于H₂O和D₂O的缓冲溶液中对鲑鱼降钙素和依降钙素胰蛋白酶消化产生的肽片段进行了毛细管电泳（CE）。发现重水中的分析优于H₂O中的分析，尤其是在pH或pD为7.93时。通过对依降钙素消化物进行一次CE运行，我们还能够收获足够量的三种纯裂解肽，以通过蛋白质测序确定每个氨基酸残基。CE洗脱顺序与根据每个肽计算的净电荷预测的顺序一致。
