Camilleri P, Okafo G N, Southan C
SmithKline Beecham Pharmaceuticals, Research and Development, The Frythe, Welwyn, Herts, United Kingdom.
Anal Biochem. 1991 Jul;196(1):178-82. doi: 10.1016/0003-2697(91)90136-h.
In this study we have explored the behaviour of peptides after capillary electrophoresis (CE) followed by elution under pressure. The use of D2O- rather than H2O-based buffer solutions appears to restrict the diffusion of peptides after CE, resulting in little loss of resolution when peptides are eluted by dynamic flow. In this paper we present results showing that a simple two-step process, involving CE at a low voltage, switching off the power supply, and connecting the fused capillary at the anode end to a syringe pump for dynamic flow, can retain separation characteristics and can be used for the isolation of picomole quantities of peptides for sequence determination.
在本研究中，我们探究了肽段在毛细管电泳（CE）后进行压力洗脱时的行为。使用基于重水（D₂O）而非水（H₂O）的缓冲溶液似乎能够限制毛细管电泳后肽段的扩散，从而在通过动态流动洗脱肽段时分辨率损失极小。在本文中，我们展示的结果表明，一个简单的两步过程，即先在低电压下进行毛细管电泳，关闭电源，然后将阳极端的熔融毛细管连接到注射泵进行动态流动，能够保留分离特性，并且可用于分离皮摩尔量的肽段以进行序列测定。
