Marques Ana P G C, Oliveira Rui S, Rangel António O S S, Castro Paula M L
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
Chemosphere. 2006 Nov;65(7):1256-63. doi: 10.1016/j.chemosphere.2006.03.022. Epub 2006 May 2.
Solanum nigrum was found to proliferate in sediments with high levels of metal pollution. The effect of Zn on plant growth and tissue metal accumulation was assessed. The response of the plant to the inoculation with four different isolates of arbuscular mycorrhizal fungi (AMF) (Glomus sp. BEG140, Glomus claroideum, Glomus mosseae and Glomus intraradices) was studied. While the isolates of AMF did not have a significant (P<0.05) influence on mycorrhizal colonisation, increasing Zn concentration to high levels (500 and 1000 mgkg(-1)) induced significant (P<0.05) decrease of the AMF colonisation. In general, the presence of AMF did not affect the growth and biomass of S. nigrum individuals. However, the level of metal in the matrix affected S. nigrum growth; plants grown at 100 mgkg(-1) had significantly (P<0.05) lower leaf, stem, root and total biomass than control ones and plants growing at 500 and 1000 mgkg(-1) had the significantly (P<0.05) lowest biomass. Plants inoculated with the AMF G. claroideum and G. intraradices presented significantly (P<0.05) higher Zn accumulation in all plant tissues. In general, the stem tissues had the higher Zn content while the leaves registered the lowest values, which indicate a high translocation of the metal. AMF inoculation had no significant (P<0.05) influence on the metal translocation within the plant. This study suggests that inoculation with the AMF G. claroideum or G. intraradices, can enhance the Zn accumulation in the tissues of S. nigrum, not affecting the plant translocation capacities.
发现龙葵在金属污染水平较高的沉积物中大量繁殖。评估了锌对植物生长和组织金属积累的影响。研究了该植物对接种四种不同丛枝菌根真菌（AMF）分离株（Glomus sp. BEG140、Glomus claroideum、Glomus mosseae和Glomus intraradices）的反应。虽然AMF分离株对菌根定殖没有显著（P<0.05）影响，但将锌浓度提高到高水平（500和1000 mgkg(-1)）会导致AMF定殖显著（P<0.05）下降。总体而言，AMF的存在不影响龙葵个体的生长和生物量。然而，基质中的金属水平影响龙葵的生长；在100 mgkg(-1)下生长的植物的叶、茎、根和总生物量显著（P<0.05）低于对照植物，而在500和1000 mgkg(-1)下生长的植物的生物量显著（P<0.05）最低。接种AMF Glomus claroideum和Glomus intraradices的植物在所有植物组织中的锌积累显著（P<0.05）更高。总体而言，茎组织中的锌含量较高，而叶中的锌含量最低，这表明金属的高转运。AMF接种对植物体内的金属转运没有显著（P<0.05）影响。这项研究表明，接种AMF Glomus claroideum或Glomus intraradices可以增强龙葵组织中的锌积累，而不影响植物的转运能力。
