Mencacci Antonella, Cenci Elio, Repetto Antonella, Mazzolla Rosanna, Bistoni Francesco, Aversa Franco, Aloisi Teresa, Vecchiarelli Anna
Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
J Infect. 2006 Dec;53(6):e259-64. doi: 10.1016/j.jinf.2006.03.016. Epub 2006 May 2.
A multidrug-resistant Pseudomonas aeruginosa (r-Pa) was isolated from a lethal case of sepsis in a bone marrow transplant recipient. Genotypic analysis of P. aeruginosa isolates demonstrated that sepsis was secondary to gut colonization. The interactions between r-Pa and patient's neutrophils were studied. The results indicate that: (1) the patient's neutrophil killing activity and nitric oxide production against r-Pa or drug sensitive P. aeruginosa (s-Pa) were profoundly impaired; (2) r-Pa cells, but not s-Pa cells or their filtered culture supernatants, induced necrosis of healthy donor neutrophils. Neutrophil necrosis emerges as a remarkable event in the pathogenesis of P. aeruginosa sepsis.
一株多重耐药铜绿假单胞菌（r-Pa）从一名骨髓移植受者的致死性败血症病例中分离得到。对铜绿假单胞菌分离株的基因分型分析表明，败血症继发于肠道定植。对r-Pa与患者中性粒细胞之间的相互作用进行了研究。结果表明：（1）患者中性粒细胞对r-Pa或药物敏感铜绿假单胞菌（s-Pa）的杀伤活性及一氧化氮产生均严重受损；（2）r-Pa菌细胞可诱导健康供体中性粒细胞坏死，而s-Pa菌细胞及其过滤后的培养上清液则无此作用。中性粒细胞坏死在铜绿假单胞菌败血症发病机制中是一个显著事件。
