EIF3S3的过表达促进癌细胞生长。
Overexpression of EIF3S3 promotes cancer cell growth.
作者信息
Savinainen Kimmo J, Helenius Merja A, Lehtonen Heli J, Visakorpi Tapio
机构信息
Laboratory of Cancer Genetics, Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere FIN-33014, Finland.
出版信息
Prostate. 2006 Aug 1;66(11):1144-50. doi: 10.1002/pros.20452.
BACKGROUND
Amplification and overexpression of EIF3S3 gene has been demonstrated in breast and prostate cancer. Here, our goal was to study the effect of EIF3S3 on cell growth.
METHODS
The effect of EIF3S3 on growth of NIH 3T3 murine fibroblasts as well as breast (SK-Br-3 and ZR-75-1) and prostate (PC-3 and LNCaP) cancer cell lines was examined by using transfection with inducible pTet-Off system and siRNAs.
RESULTS
NIH 3T3 cells with overexpression of EIF3S3 grew significantly faster than cells transfected with empty vector and survived longer when grown in soft agar. The EIF3S3 overexpression was associated with increased fraction of cells in S-phase and with phosphorylation of retinoblastoma (Rb) protein. siRNA treatment inhibited significantly (P = 0.0022) the growth of all breast and prostate cancer cell lines studied.
CONCLUSIONS
The results suggest that EIF3S3 regulates cell growth and viability, and that overexpression of the gene may provide growth advantage to the cancer cells.
背景
EIF3S3基因的扩增和过表达已在乳腺癌和前列腺癌中得到证实。在此，我们的目标是研究EIF3S3对细胞生长的影响。
方法
通过使用可诱导的pTet-Off系统和小干扰RNA（siRNAs）转染，检测EIF3S3对NIH 3T3小鼠成纤维细胞以及乳腺癌（SK-Br-3和ZR-75-1）和前列腺癌（PC-3和LNCaP）细胞系生长的影响。
结果
EIF3S3过表达的NIH 3T3细胞比用空载体转染的细胞生长明显更快，并且在软琼脂中生长时存活时间更长。EIF3S3过表达与S期细胞比例增加以及视网膜母细胞瘤（Rb）蛋白磷酸化有关。小干扰RNA处理显著抑制（P = 0.0022）了所有研究的乳腺癌和前列腺癌细胞系的生长。
结论
结果表明EIF3S3调节细胞生长和活力，并且该基因的过表达可能为癌细胞提供生长优势。
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