Department of Horticulture, Iowa State University, Ames, Iowa 50011.
Plant Physiol. 1992 Sep;100(1):164-9. doi: 10.1104/pp.100.1.164.
Sequences homologous to a potato cathepsin D inhibitor cDNA, p749, were identified in the genomic DNA of tomato (Lycopersicon esculentum) and of two non-tuber-bearing potato species (Solanum etuberosum and S. brevidens) by means of Southern blot analysis. The expression of these p749 genes in leaves was induced at the RNA level in response to wounding. High levels of p749 transcripts were detected in polyadenylated RNA extracted from locally wounded leaves 12 h after wounding. Systemic induction of the cathepsin D inhibitor gene also occurred in nonwounded leaves of wounded plants. Both potato and tomato leaves treated with the oligosaccharide chitosan showed an induced accumulation of p749 transcripts. Even though the cathepsin D inhibitor genes from tomato and from non-tuber-bearing potato species are wound inducible, they could not be induced in leaf explants cultured on medium containing very high concentrations of sucrose. Only leaf explants from the tuber-bearing potato (S. tuberosum) accumulated p749 transcripts when cultured on high sucrose medium. A sequence related to the 22-kD potato proteinase inhibitor cDNA, p34021, was identified in tomato by means of genomic Southern blot analysis. Northern blot hybridization showed that p34021 transcripts accumulated in potato (S. tuberosum) leaf explants, but not in tomato explants, when cultured on high sucrose medium. This study demonstrates that the expression of a potato cathepsin D inhibitor gene in tomato and in non-tuber-bearing potato species is wound inducible, but not sucrose inducible.
通过 Southern 印迹分析，在番茄（Lycopersicon esculentum）和两个无块茎马铃薯种（Solanum etuberosum 和 S. brevidens）的基因组 DNA 中鉴定出与马铃薯组织蛋白酶 D 抑制剂 cDNA p749 同源的序列。这些 p749 基因在叶片中的表达在 RNA 水平上受到损伤的诱导。在受伤后 12 小时从局部受伤叶片中提取的多聚腺苷酸化 RNA 中检测到高水平的 p749 转录物。在受伤植物的未受伤叶片中也发生了组织蛋白酶 D 抑制剂基因的系统诱导。用寡糖壳聚糖处理的马铃薯和番茄叶片均表现出 p749 转录物的诱导积累。尽管来自番茄和无块茎马铃薯种的组织蛋白酶 D 抑制剂基因是诱导性的，但它们不能在含有非常高浓度蔗糖的培养基上培养的叶片外植体中诱导。只有在高蔗糖培养基上培养的块茎马铃薯（S. tuberosum）叶片外植体才会积累 p749 转录物。通过基因组 Southern 印迹分析，在番茄中鉴定出与 22-kD 马铃薯蛋白酶抑制剂 cDNA p34021 相关的序列。Northern 印迹杂交显示，当在高蔗糖培养基上培养时，p34021 转录物在马铃薯（S. tuberosum）叶片外植体中积累，但在番茄外植体中不积累。这项研究表明，马铃薯组织蛋白酶 D 抑制剂基因在番茄和无块茎马铃薯种中的表达是诱导性的，但不是蔗糖诱导性的。
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