Molnár A, Lovas A, Bánfalvi Z, Lakatos L, Polgár Z, Horváth S
Agricultural Biotechnology Center, Gödöllö, Hungary.
Plant Mol Biol. 2001 Jun;46(3):301-11. doi: 10.1023/a:1010649503229.
The molecular basis of the differential expression of the GM7-type metallocarboxypeptidase inhibitor (MCPI) genes in tuberizing (StMCPI) and non-tuberizing Solanum species (SbMCPI) was investigated. It was shown that the StMCPI is encoded by a gene family in Solanum tuberosum (potato), but SbMCPI might be a single-copy gene in the non-tuberizing species Solanum brevidens. The StMCPI promoter shows evolutionary relatedness to the S. brevidens-derived SbMCPI and to the fruit-specific tomato promoter 2A11. Both StMCPI and SbMCPI promoter regions were able to confer tuber- and berry-specific expression for the beta-glucuronidase reporter gene in potato suggesting that the difference in MCPI gene expression is in trans regulatory factors between the tuberizing and the non-tuberizing Solanum species. The MCPI promoters did not respond to metabolic, environmental or hormonal signals in leaves. Thus, the MCPI genes are regulated in a different way than the other known tuber-specific genes and potentially are suitable for biotechnological application in potato to provide specific transgene expression in tuber and berry.
研究了GM7型金属羧肽酶抑制剂（MCPI）基因在块茎形成的茄属植物（StMCPI）和不形成块茎的茄属植物（SbMCPI）中差异表达的分子基础。结果表明，StMCPI由马铃薯（Solanum tuberosum）中的一个基因家族编码，但SbMCPI在不形成块茎的物种短柄茄（Solanum brevidens）中可能是单拷贝基因。StMCPI启动子与源自短柄茄的SbMCPI以及果实特异性番茄启动子2A11具有进化相关性。StMCPI和SbMCPI启动子区域都能够在马铃薯中赋予β-葡萄糖醛酸酶报告基因块茎和浆果特异性表达，这表明MCPI基因表达的差异在于块茎形成和不形成块茎的茄属物种之间的反式调节因子。MCPI启动子在叶片中对代谢、环境或激素信号没有反应。因此，MCPI基因的调控方式与其他已知的块茎特异性基因不同，并且可能适用于马铃薯的生物技术应用，以在块茎和浆果中提供特异性转基因表达。
