Hashida R, Chiba K, Yamatsu I, Katayama K
Eisai Co., Ltd., Tsukuba Research Laboratories, Ibaraki, Japan.
Agents Actions. 1991 Sep;34(1-2):242-3. doi: 10.1007/BF01993292.
To elucidate the role of collagenase in tissue destruction in chronic inflammation (e.g., in rheumatoid arthritis), we established a novel air pouch model in rats. Ten ml of air was injected subcutaneously on the dorsa of F344 rats, and 24 hours thereafter, Freund's complete adjuvant was injected into the air pouch. A remarkably high activity of trypsin-activated collagenase was detected dose-dependently in the pouch fluid at 8 days after the challenge injection. Concomitantly with the increase of collagenase activity, the weight of granuloma formed in the pouch decreased, suggesting that collagenase participates in the resorption of granuloma tissues.
为了阐明胶原酶在慢性炎症（如类风湿性关节炎）组织破坏中的作用，我们在大鼠中建立了一种新型气袋模型。将10毫升空气皮下注射到F344大鼠的背部，24小时后，将弗氏完全佐剂注射到气袋中。在激发注射后8天，在袋液中剂量依赖性地检测到胰蛋白酶激活的胶原酶的显著高活性。伴随着胶原酶活性的增加，气袋中形成的肉芽肿重量减轻，这表明胶原酶参与了肉芽肿组织的吸收。
