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从大麦中纯化一种与感染相关的细胞外过氧化物酶。

Purification of an infection-related, extracellular peroxidase from barley.

机构信息

Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312.

出版信息

Plant Physiol. 1992 Sep;100(1):397-402. doi: 10.1104/pp.100.1.397.

DOI:10.1104/pp.100.1.397
PMID:16652974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1075564/
Abstract

Increases in two extracellular peroxidases were observed following inoculation of barley (Hordeum vulgare L.) with the powdery mildew pathogen (Erysiphe graminis DC.: Fr. f. sp. hordei Em. Marchal). The more prominent isozyme, P8.5, was purified from intercellular wash fluids by acetone precipitation, ion-exchange chromatography, isoelectric focusing, and gel filtration. Purified P8.5 is a heme-containing, glycoprotein with a M(r) of 35,000. It has eight cysteine residues. A highly specific, high-titer antiserum to deglycosylated P8.5 was produced.

摘要

接种大麦白粉病菌（Erysiphe graminis DC.：Fr. f. sp. hordei Em. Marchal）后，观察到两种细胞外过氧化物酶增加。同工酶 P8.5 从细胞间洗涤液中通过丙酮沉淀、离子交换层析、等电聚焦和凝胶过滤进行纯化。纯化的 P8.5 是一种含有血红素的糖蛋白，Mr 为 35000。它有 8 个半胱氨酸残基。产生了针对去糖基化 P8.5 的高度特异性、高滴度抗血清。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11f8/1075564/e93acc1f5a07/plntphys00709-0410-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11f8/1075564/565072b96c72/plntphys00709-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11f8/1075564/d733a195d12d/plntphys00709-0409-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11f8/1075564/e93acc1f5a07/plntphys00709-0410-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11f8/1075564/565072b96c72/plntphys00709-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11f8/1075564/d733a195d12d/plntphys00709-0409-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11f8/1075564/e93acc1f5a07/plntphys00709-0410-a.jpg

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本文引用的文献

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Soluble and Bound Apoplastic Activity for Peroxidase, beta-d-Glucosidase, Malate Dehydrogenase, and Nonspecific Arylesterase, in Barley (Hordeum vulgare L.) and Oat (Avena sativa L.) Primary Leaves.大麦（Hordeum vulgare L.）和燕麦（Avena sativa L.）初生叶片中过氧化物酶、β-D-葡萄糖苷酶、苹果酸脱氢酶和非特异性芳基酯酶的可溶和束缚质外体活性。
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Covalent structure of turnip peroxidase 7. Cyanogen bromide fragments, complete structure and comparison to horseradish peroxidase C.
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Acquired Resistance in Barley (The Resistance Mechanism Induced by 2,6-Dichloroisonicotinic Acid Is a Phenocopy of a Genetically Based Mechanism Governing Race-Specific Powdery Mildew Resistance).大麦中的获得性抗性（2,6-二氯异烟酸诱导的抗性机制是一种基于基因的控制小种特异性白粉病抗性机制的表型模拟）
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