Bengtsson Therése, Åhman Inger, Manninen Outi, Reitan Lars, Christerson Therese, Due Jensen Jens, Krusell Lene, Jahoor Ahmed, Orabi Jihad
Department of Plant Breeding, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Boreal Plant Breeding Ltd., Jokioinen, Finland.
Front Plant Sci. 2017 Nov 14;8:1954. doi: 10.3389/fpls.2017.01954. eCollection 2017.
The powdery mildew fungus, f. sp. is a worldwide threat to barley ( L. ssp. ) production. One way to control the disease is by the development and deployment of resistant cultivars. A genome-wide association study was performed in a Nordic spring barley panel consisting of 169 genotypes, to identify marker-trait associations significant for powdery mildew. Powdery mildew was scored during three years (2012-2014) in four different locations within the Nordic region. There were strong correlations between data from all locations and years. In total four QTLs were identified, one located on chromosome 4H in the same region as the previously identified locus and three on chromosome 6H. Out of these three QTLs identified on chromosome 6H, two are in the same region as previously reported QTLs for powdery mildew resistance, whereas one QTL appears to be novel. The top NCBI BLASTn hit of the SNP markers within the novel QTL predicted the responsible gene to be the 26S proteasome regulatory subunit, RPN1, which is required for innate immunity and powdery mildew-induced cell death in . The results from this study have revealed SNP marker candidates that can be exploited for use in marker-assisted selection and stacking of genes for powdery mildew resistance in barley.
白粉病菌，f. sp.，是对大麦（L. ssp.）生产的全球性威胁。控制该病害的一种方法是培育和推广抗性品种。在一个由169个基因型组成的北欧春大麦群体中进行了全基因组关联研究，以鉴定对白粉病具有显著标记 - 性状关联的位点。在北欧地区的四个不同地点，于三年（2012 - 2014年）期间对白粉病进行了评分。所有地点和年份的数据之间存在很强的相关性。总共鉴定出四个QTL，一个位于4H染色体上与先前鉴定的位点相同的区域，另外三个位于6H染色体上。在6H染色体上鉴定出的这三个QTL中，有两个与先前报道的白粉病抗性QTL位于同一区域，而其中一个QTL似乎是新的。新QTL内SNP标记在NCBI BLASTn中的最佳匹配预测负责基因是26S蛋白酶体调节亚基RPN1，它是植物先天免疫和白粉病诱导的细胞死亡所必需的。本研究结果揭示了可用于大麦白粉病抗性基因的标记辅助选择和基因累加的SNP标记候选物。
